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p21 Waf1/Cip1 (DCS60) Mouse Monoclonal Antibody #2946

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  • WB
  • IHC

    Product Specifications

    REACTIVITY H Mk
    SENSITIVITY Endogenous
    MW (kDa) 21
    Source/Isotype Mouse IgG2a
    Application Key:
    • WB-Western Blotting 
    • IHC-Immunohistochemistry 
    Species Cross-Reactivity Key:
    • H-Human 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:2000
    Immunohistochemistry (Paraffin) 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    p21 Waf1/Cip1 (DCS60) Mouse Monoclonal Antibody detects endogenous levels of total p21 protein. This antibody does not cross-react with other cdk inhibitors.

    Species Reactivity:

    Human, Monkey

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with recombinant human p21 corresponding to the amino-terminal portion of p21.

    Background

    The tumor suppressor protein p21 Waf1/Cip1 acts as an inhibitor of cell cycle progression. It functions in stoichiometric relationships forming heterotrimeric complexes with cyclins and cyclin-dependent kinases. In association with CDK2 complexes, it serves to inhibit kinase activity and block progression through G1/S (1). However, p21 may also enhance assembly and activity in complexes of CDK4 or CDK6 and cyclin D (2). The carboxy-terminal region of p21 is sufficient to bind and inhibit PCNA, a subunit of DNA polymerase, and may coordinate DNA replication with cell cycle progression (3). Upon UV damage or during cell cycle stages when cdc2/cyclin B or CDK2/cyclin A are active, p53 is phosphorylated and upregulates p21 transcription via a p53-responsive element (4). Protein levels of p21 are downregulated through ubiquitination and proteasomal degradation (5).

    Alternate Names

    CAP20; CDK-interacting protein 1; CDK-interaction protein 1; CDKN1; CDKN1A; CDN1A; CIP1; cyclin dependent kinase inhibitor 1A; Cyclin-dependent kinase inhibitor 1; cyclin-dependent kinase inhibitor 1A (p21, Cip1); DNA synthesis inhibitor; MDA-6; MDA6; melanoma differentiation associated protein 6; Melanoma differentiation-associated protein 6; p21; p21CIP1; p21Cip1/Waf1; PIC1; SDI1; WAF1; wild-type p53-activated fragment 1

    For Research Use Only. Not for Use in Diagnostic Procedures.
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