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p21 Waf1/Cip1 (F7S9M) Rabbit Monoclonal Antibody #39375

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  • WB
  • IP
  • IHC

    Product Specifications

    REACTIVITY M
    SENSITIVITY Endogenous
    MW (kDa) 21
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    • IHC-Immunohistochemistry 
    Species Cross-Reactivity Key:
    • M-Mouse 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:100
    Immunohistochemistry (Paraffin) 1:400 - 1:1600

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    p21 Waf1/Cip1 (F7S9M) Rabbit Monoclonal Antibody recognizes endogenous levels of total p21 Waf1/Cip1 protein. This antibody detects approximately 15 kDa and 105 kDa proteins of unknown identity in some cell lines.

    Species Reactivity:

    Mouse

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro11 of mouse p21 Waf1/Cip1 protein.

    Background

    The tumor suppressor protein p21 Waf1/Cip1 acts as an inhibitor of cell cycle progression. It functions in stoichiometric relationships forming heterotrimeric complexes with cyclins and cyclin-dependent kinases. In association with CDK2 complexes, it serves to inhibit kinase activity and block progression through G1/S (1). However, p21 may also enhance assembly and activity in complexes of CDK4 or CDK6 and cyclin D (2). The carboxy-terminal region of p21 is sufficient to bind and inhibit PCNA, a subunit of DNA polymerase, and may coordinate DNA replication with cell cycle progression (3). Upon UV damage or during cell cycle stages when cdc2/cyclin B or CDK2/cyclin A are active, p53 is phosphorylated and upregulates p21 transcription via a p53-responsive element (4). Protein levels of p21 are downregulated through ubiquitination and proteasomal degradation (5).

    Alternate Names

    CAP; CAP20; CDK; CDK-interacting protein 1; CDKI; Cdkn; Cdkn1; Cdkn1a; CDN1A; Cip1; Cyclin-dependent kinase inhibitor 1; cyclin-dependent kinase inhibitor 1A (P21); mda; mda6; Melanoma differentiation-associated protein; p21; p21<WAF1/CIP1>; p21C; p21Cip1; p21W; p21WAF; SDI1; Waf; Waf1

    For Research Use Only. Not for Use in Diagnostic Procedures.
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