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PhosphoSitePlus® Resource

  • Additional protein information
  • Analytical tools


Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Phospho-p90RSK (Thr359) (D1E9) Rabbit mAb 8753 20 µl
Western Blotting Immunoprecipitation Immunohistochemistry Immunofluorescence
H Mk 90 Rabbit IgG
Phospho-p90RSK (Ser380) (D3H11) Rabbit mAb 11989 20 µl
Western Blotting Immunohistochemistry Immunofluorescence
H M R Mk Mi 90 Rabbit IgG
Phospho-p90RSK (Thr573) Antibody 9346 20 µl
Western Blotting Immunofluorescence
H M R 90 Rabbit 
RSK1/RSK2/RSK3 (32D7) Rabbit mAb 9355 20 µl
Western Blotting Immunoprecipitation Immunofluorescence
H M R Mk GP 90 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
Western Blotting

Product Description

The p90RSK Antibody Sampler Kit provides an economical means of evaluating levels of p90RSK protein phosphorylated at specific sites, as well as total p90RSK protein levels. The kit contains enough primary and secondary antibody to perform two Western blot experiments.

Specificity / Sensitivity

RSK1/RSK2/RSK3 (32D7) Rabbit mAb detects endogenous levels of total RSK1-3 proteins. Each activation state monoclonal antibody recognizes its specific target; some cross-reactivity with related phosphorylated RSK proteins may occur.

Source / Purification

The phospho-specific polyclonal antibody are produced by immunizing animals with a synthetic phosphopeptide corresponding to residue Thr573 of human p90RSK. Polyclonal antibodies are purified by protein A and peptide affinity chromatography. The rabbit monoclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr356 of human p90RSK3 protein, Ser377 of human p90RSK and a nonphosphorylated peptide surrounding Asp659 of human p90RSK for total RSK1/RSK2/RSK3.

The 90 kDa ribosomal S6 kinases (RSK1-4) are a family of widely expressed Ser/Thr kinases characterized by two nonidentical, functional kinase domains (1) and a carboxy-terminal docking site for extracellular signal-regulated kinases (ERKs) (2). Several sites both within and outside of the RSK kinase domain, including Ser380, Thr359, Ser363, and Thr573, are important for kinase activation (3). RSK1-3 are activated via coordinated phosphorylation by MAPKs, autophosphorylation, and phosphoinositide-3-OH kinase (PI3K) in response to many growth factors, polypeptide hormones, and neurotransmitters (3).

1.  Fisher, T.L. and Blenis, J. (1996) Mol Cell Biol 16, 1212-9.

2.  Smith, J.A. et al. (1999) J Biol Chem 274, 2893-8.

3.  Dalby, K.N. et al. (1998) J Biol Chem 273, 1496-505.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.

p90RSK Antibody Sampler Kit