View Featured Offers >>
28002
Pancreatic Marker IHC Antibody Sampler Kit
Primary Antibodies
Antibody Sampler Kit

Pancreatic Marker IHC Antibody Sampler Kit #28002

Reviews ()
Citations (0)
Pancreatic Marker IHC Antibody Sampler Kit: Image 1
Western blot analysis of extracts from various cell lines using CD200 (E5I9V) XP® Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).
Pancreatic Marker IHC Antibody Sampler Kit: Image 2
Immunohistochemical analysis of paraffin-embedded human pancreas, showing the staining of β cells, using Insulin (C27C9) Rabbit mAb.
Pancreatic Marker IHC Antibody Sampler Kit: Image 3
Western blot analysis of extracts from various cell lines, using Pan-Keratin (C11) Mouse mAb.
Pancreatic Marker IHC Antibody Sampler Kit: Image 4
Immunohistochemical analysis of paraffin-embedded human pancreas, showing staining of β-cells, using C-Peptide Antibody.
Pancreatic Marker IHC Antibody Sampler Kit: Image 5
Western blot analysis of extracts from various cell lines using NKX6.1 (D8O4R) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).
Pancreatic Marker IHC Antibody Sampler Kit: Image 6
Western blot analysis of extracts from various cell lines using Pax6 (D3A9V) XP® Rabbit mAb (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). As expected, A172 and MCF7 cells are negative.
Pancreatic Marker IHC Antibody Sampler Kit: Image 7
After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.
Pancreatic Marker IHC Antibody Sampler Kit: Image 8
After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO* is added and emits light during enzyme catalyzed decomposition.
Pancreatic Marker IHC Antibody Sampler Kit: Image 9
Immunohistochemical analysis of paraffin-embedded human pancreas using Proglucagon (D16G10) XP® Rabbit mAb.
Pancreatic Marker IHC Antibody Sampler Kit: Image 10
Immunoprecipitation of CD200 from SK-MEL-28 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is CD200 (E5I9V) XP® Rabbit mAb. Western blot analysis was performed using CD200(E5I9V) XP® Rabbit mAb as the primary antibody and Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb (HRP Conjugate) #5127 as the secondary antibody.
Pancreatic Marker IHC Antibody Sampler Kit: Image 11
Immunohistochemical anaylsis of paraffin-embedded mouse pancreas, showing staining of β cells, using Insulin (C27C9) Rabbit mAb.
Pancreatic Marker IHC Antibody Sampler Kit: Image 12
Immunohistochemical analysis of paraffin-embedded human transitional epithelial carcinoma (bladder), using Pan-Keratin (C11) Mouse mAb.
Pancreatic Marker IHC Antibody Sampler Kit: Image 13
Immunohistochemical analysis of paraffin-embedded mouse pancreas, showing staining of β-cells, using C-Peptide Antibody.
Pancreatic Marker IHC Antibody Sampler Kit: Image 14
Immunohistochemical analysis of paraffin-embedded mouse pancreas using NKX6.1 (D8O4R) Rabbit mAb.
Pancreatic Marker IHC Antibody Sampler Kit: Image 15
Immunohistochemical analysis of paraffin-embedded 293 cell pellet (left, positive) or MCF7 cell pellet (right, negative) using Pax6 (D3A9V) XP® Rabbit mAb.
Pancreatic Marker IHC Antibody Sampler Kit: Image 16
Immunohistochemical analysis of paraffin-embedded mouse pancreas using Proglucagon (D16G10) XP® Rabbit mAb.
Pancreatic Marker IHC Antibody Sampler Kit: Image 17
Immunohistochemical analysis of paraffin-embedded human gastric adenocarcinoma using CD200 (E5I9V) XP® Rabbit mAb performed on the Leica® BOND Rx.
Pancreatic Marker IHC Antibody Sampler Kit: Image 18
Immunohistochemical analysis of paraffin-embedded rat pancreas, showing staining of β cells, using Insulin (C27C9) Rabbit mAb.
Pancreatic Marker IHC Antibody Sampler Kit: Image 19
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Pan-Keratin (C11) Mouse mAb.
Pancreatic Marker IHC Antibody Sampler Kit: Image 20
Immunohistochemical analysis of paraffin-embedded rat pancreas showing staining of β-cells using C-Peptide Antibody.
Pancreatic Marker IHC Antibody Sampler Kit: Image 21
Immunohistochemical analysis of paraffin-embedded human pancreas using NKX6.1 (D8O4R) Rabbit mAb.
Pancreatic Marker IHC Antibody Sampler Kit: Image 22
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Pax6 (D3A9V) XP® Rabbit mAb.
Pancreatic Marker IHC Antibody Sampler Kit: Image 23
Immunohistochemical analysis of paraffin-embedded rat pancreas using Proglucagon (D16G10) XP® Rabbit mAb.
Pancreatic Marker IHC Antibody Sampler Kit: Image 24
Immunohistochemical analysis of paraffin-embedded human ovarian serous carcinoma using CD200 (E5I9V) XP® Rabbit mAb.
Pancreatic Marker IHC Antibody Sampler Kit: Image 25
Confocal immunofluorescent analysis of rat pancreas using Insulin (C27C9) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Pancreatic Marker IHC Antibody Sampler Kit: Image 26
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Pan-Keratin (C11) Mouse mAb.
Pancreatic Marker IHC Antibody Sampler Kit: Image 27
Immunohistochemical analysis of paraffin-embedded β-TC-6 (left) and mIMCD3 (right) cell pellets using NKX6.1 (D8O4R) Rabbit mAb.
Pancreatic Marker IHC Antibody Sampler Kit: Image 28
Immunohistochemical analysis of paraffin-embedded human endometrioid adenocarcinoma using Pax6 (D3A9V) XP® Rabbit mAb.
Pancreatic Marker IHC Antibody Sampler Kit: Image 29
Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma (left), prostate carcinoma (middle), or lymph node (right) using CD200 (E5I9V) XP® Rabbit mAb (top) or CD200 Rabbit mAb (bottom). These two antibodies detect independent, unique epitopes on human CD200. The similar staining patterns obtained with both antibodies help to confirm the specificity of the staining.
Pancreatic Marker IHC Antibody Sampler Kit: Image 30
Confocal immunofluorescent analysis of β-TC-6 (left) and C2C12 cells (right) using Insulin (C27C9) Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Pancreatic Marker IHC Antibody Sampler Kit: Image 31
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma, using Pan-Keratin (C11) Mouse mAb.
Pancreatic Marker IHC Antibody Sampler Kit: Image 32
Confocal immunofluorescent analysis of mouse pancreas using C-Peptide Antibody (green). Blue pseudocolor = DRAQ5 (fluorescent DNA dye).
Pancreatic Marker IHC Antibody Sampler Kit: Image 33
Confocal immunofluorescent analysis of β-TC-6 (positive; left) or mIMCD-3 (negative; right) cells, using NKX6.1 (D8O4R) Rabbit mAb (green). Actin filaments were labeled with DyLightTM 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Pancreatic Marker IHC Antibody Sampler Kit: Image 34
Immunohistochemical analysis of paraffin-embedded normal human pancreas using Pax6 (D3A9V) XP® Rabbit mAb.
Pancreatic Marker IHC Antibody Sampler Kit: Image 35
Confocal immunofluorescent analysis of mouse pancreas using Proglucagon (D16G10) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Pancreatic Marker IHC Antibody Sampler Kit: Image 36
Immunohistochemical analysis of paraffin-embedded human placenta using CD200 (E5I9V) XP® Rabbit mAb.
Pancreatic Marker IHC Antibody Sampler Kit: Image 37
Flow cytometric analysis of C2C12 cells (blue, negative) and β-TC-6 cells (green, positive) using Insulin (C27C9) Rabbit mAb (solid lines) or a concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.
Pancreatic Marker IHC Antibody Sampler Kit: Image 38
Immunohistochemical analysis of paraffin-embedded H358 xenograft, using Pan-Keratin (C11) Mouse mAb.
Pancreatic Marker IHC Antibody Sampler Kit: Image 39
Confocal immunofluorescent analysis of β-TC-6 cells (left) and HeLa cells (right) using C-Peptide Antibody (green). Actin filaments have been labeled with DY-555 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Pancreatic Marker IHC Antibody Sampler Kit: Image 40
Flow cytometric analysis of mIMCD-3 (blue) and β-TC-6 (green) cells using NKX6.1 (D8O4R) Rabbit mAb. Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.
Pancreatic Marker IHC Antibody Sampler Kit: Image 41
Immunohistochemical analysis of paraffin-embedded normal human stomach using Pax6 (D3A9V) XP® Rabbit mAb.
Pancreatic Marker IHC Antibody Sampler Kit: Image 42
Immunohistochemical analysis of paraffin-embedded human ductal breast carcinoma using CD200 (E5I9V) XP® Rabbit mAb (left) compared to concentration matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right).
Pancreatic Marker IHC Antibody Sampler Kit: Image 43
Immunohistochemical analysis of paraffin-embedded human ductal breast carcinoma using Pan-Keratin (C11) mouse mAb.
Pancreatic Marker IHC Antibody Sampler Kit: Image 44
Confocal immunofluorescent analysis of 293T cells (left, positive) and MCF7 cells (right, negative) using Pax6 (D3A9V) XP® Rabbit mAb (green). Actin filaments were labeled with DyLight 554 Phalloidin #13054 (red).
Pancreatic Marker IHC Antibody Sampler Kit: Image 45
Immunohistochemical analysis of paraffin-embedded SK-MEL-28 cell pellet (left, positive) or DU 145 cell pellet (right, negative) using CD200 (E5I9V) XP® Rabbit mAb.
Pancreatic Marker IHC Antibody Sampler Kit: Image 46
Immunohistochemical analysis of paraffin-embedded human endometrioid adenocarcinoma using Pan-Keratin (C11) mouse mAb.
Pancreatic Marker IHC Antibody Sampler Kit: Image 47
Immunohistochemical analysis of paraffin-embedded human prostate adenocarcinoma using Pan-Keratin (C11) mouse mAb.
Pancreatic Marker IHC Antibody Sampler Kit: Image 48
Confocal immunofluorescent analysis of HeLa cells using Pan-Keratin (C11) Mouse mAb (green). Blue pseudocolor= DRAQ5® #4084 (fluorescent DNA dye).
Pancreatic Marker IHC Antibody Sampler Kit: Image 49
Flow cytometric analysis of A-431 cells using Pan-Keratin (C11) Mouse mAb (solid line) versus a concentration-matched Mouse (G3A1) mAb IgG1 Isotype Control #5415 (dashed line). Anti-mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4408 was used as a secondary antibody.
To Purchase # 28002
Cat. # Size Qty. Price
28002T
1 Kit  (7 x 20 microliters)
$ 610

Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Insulin (C27C9) Rabbit mAb 3014 20 µl
  • IHC
  • IF
  • F
H M R Rabbit IgG
Proglucagon (D16G10) XP® Rabbit mAb 8233 20 µl
  • IHC
  • IF
H M R Rabbit IgG
CD200 (E5I9V) XP® Rabbit mAb 23451 20 µl
  • WB
  • IP
  • IHC
H 45-50 Rabbit IgG
Pax6 (D3A9V) XP® Rabbit mAb 60433 20 µl
  • WB
  • IP
  • IHC
  • IF
H 50 Rabbit IgG
NKX6.1 (D8O4R) Rabbit mAb 54551 20 µl
  • WB
  • IP
  • IHC
  • IF
  • F
H M R 44, 46 Rabbit IgG
C-Peptide Antibody 4593 20 µl
  • IHC
  • IF
H M R 4 Rabbit 
Pan-Keratin (C11) Mouse mAb 4545 20 µl
  • WB
  • IHC
  • IF
  • F
H M R Mk 46-58 Mouse IgG1
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
  • WB
Rab Goat 
Anti-mouse IgG, HRP-linked Antibody 7076 100 µl
  • WB
M Horse 

Product Description

The Pancreatic Marker IHC Antibody Sampler Kit provides a useful selection of markers to distinguish pancreatic cell types that perform important functions to maintain glucose homeostasis. The kit also includes antibodies that differentiate pancreatic tumor subtypes. The sampler kit is designed for use on formalin-fixed, paraffin-embedded tissue samples.

Specificity / Sensitivity

Each antibody in the Pancreatic Marker IHC Antibody Sampler Kit detects endogenous levels of its target protein. Pan-Keratin (C11) Mouse mAb detects total keratin 4, 5, 6, 8, 10, 13, and 18. The antibody does not cross-react with other keratins. NKX6.1 (D8O4R) Rabbit mAb recognizes total NKX6.1 protein. Note: Some non-specific staining of squamous epithelium has been observed by IHC. It is unclear if the antibody works on human samples in western blotting but is predicted to based on 100% sequence homology.

Source / Purification

Monoclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to Val42 of human insulin, Ala137 of human proglucagon, residues near the carboxy terminus of human Pax6, and to human CD200 recombinant protein. Pan-Keratin (C11) Mouse mAb (isotype: IgG1) is produced by immunizing a BALB/c mouse with a cytoskeleton preparation from A-431 cells. Polyclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to the sequence of human glucagon, and the sequence of human C-peptide. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Insulin is a hormone that is produced and released from pancreatic β cells through a glucose sensing pathway. Proinsulin is the precursor molecule to insulin and is processed prior to its secretion. Insulin is composed of A-peptide and B-peptide which are joined by a disulfide bond. The center one-third of the molecule is cleaved and released as C-peptide, which has a longer half-life than insulin (1). Antibodies to both insulin and C-peptide are useful markers for β cells.

Glucose homeostasis is regulated by a variety of hormones including glucagon. Glucagon is synthesized as the precursor molecule proglucagon and is proteolytically processed to yield the mature peptide in α cells of the pancreatic islets. Glucagon causes the release of glucose from glycogen and stimulates gluconeogenesis in the liver (2). Antibodies to glucagon and proglucagon are useful markers for pancreatic α cells.

NKX6.1 is an important transcription factor in a network of transcription factors that are critical for pancreatic β cell development and maintenance (3). Antibodies to NKX6.1 are useful markers for β cells.

Pan-Keratin and CD200 antibodies are useful to mark and differentiate some pancreatic tumor subtypes. Pan-Keratin antibodies mark epithelial cells in pancreatic adenocarcinomas while CD200 is a useful marker for neuroendocrine pancreatic tumors (4).
  1. Straub, S.G. and Sharp, G.W. (2002) Diabetes Metab Res Rev 18, 451-63.
  2. Richter, M.M. et al. (2022) Diabetes 71, 1852-1861.
  3. Habener, J.F. et al. (2005) Endocrinology 146, 1025-34.
  4. Lawlor, R.T. et al. (2019) Virchows Arch 474, 105-109.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.
All other trademarks are the property of their respective owners. Visit our Trademark Information page.