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PAR2 (D61D5) Rabbit Monoclonal Antibody

PAR2 (D61D5) Rabbit Monoclonal Antibody #6976

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Product Specifications

REACTIVITY	H
SENSITIVITY	Endogenous
MW (kDa)	32
Source/Isotype	Rabbit IgG

Application Key:

WB-Western Blotting

Species Cross-Reactivity Key:

H-Human

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

PAR2 (D61D5) Rabbit Monoclonal Antibody recognizes endogenous levels of total PAR2 protein. The major band detected runs at 32 kDa, the higher molecular weight smear is due to glycosylation.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly44 of human PAR2 protein.

Background

PAR2 (F2RL1) belongs to the PAR (Protease-activated Receptor) family of G protein-coupled receptors. These membrane receptors are activated through N-terminal cleavage of the receptor by a serine protease such as thrombin, trypsin, or matrix metalloproteinases (1,2). This cleavage exposes the ‘tethered-ligand’ fragment of the receptor, which binds to a second extracellular loop of the receptor and leads to receptor activation. PAR2 is specifically activated by trypsin or trypsin-like proteases. Activated PAR2 stimulates phosphoinositide hydrolysis and calcium mobilization, interacts with β-arrestin, and eventually leads to ERK activation (3). PAR2 expression and activation are mainly associated with inflammatory diseases (3), but may also play a role in cancer development (4,5).

Alternate Names

coagulation factor II (thrombin) receptor-like 1; Coagulation factor II receptor-like 1; F2R like trypsin receptor 1; F2RL1; G protein-coupled receptor-11; G-protein coupled receptor 11; GPR11; PAR-2; PAR2; protease-activated receptor 2; Proteinase-activated receptor 2; Proteinase-activated receptor 2, alternate cleaved 1; Proteinase-activated receptor 2, alternate cleaved 2; proteinase-activated receptor-2; Thrombin receptor-like 1

Database Links

UniProt ID: P55085

Entrez-Gene Id: 2150

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For Research Use Only. Not for Use in Diagnostic Procedures.

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