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PARIS/ZNF746 (F7U7T) Rabbit Monoclonal Antibody #17821

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  • WB
  • IP

    Product Specifications

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 95, 85
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Simple Western™ 1:50 - 1:250
    Immunoprecipitation 1:100

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    PARIS/ZNF746 (F7U7T) Rabbit Monoclonal Antibody recognizes endogenous levels of total PARIS/ZNF746 protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly435 of human PARIS/ZNF746 protein.

    Background

    Parkin-interacting substrate (PARIS), also known as Zinc finger protein 746 (ZNF746), is a tightly regulated transcriptional repressor. Structurally, PARIS/ZNF746 contains an N-terminal Krüppel-associated box (KRAB) domain and four zinc finger domains. The zinc finger domains promote DNA binding, while the KRAB domain mediates its repressor activity. PARIS/ZNF746 binds insulin response sequences (IRS) within the promoter region of peroxisome-proliferator activated receptor gamma coactivator-1 alpha (PGC-1 alpha), downregulating its expression (1,2). PGC-1 alpha is a master regulator of mitochondrial biogenesis, which is important for combating the buildup of reactive oxygen species (ROS) and maintaining cellular homeostasis. Aggregation of toxic protein species, including alpha-synuclein in Parkinson’s disease (PD), leads to ROS buildup. PGC-1 alpha overexpression has been shown to be protective in PD models. PARIS/ZNF746 regulation of PGC-1 alpha expression is mediated by PTEN induced putative kinase 1 (PINK1) and Parkin. PINK1 phosphorylates PARIS/ZNF746, leading to ubiquitination by Parkin and degradation by the proteasome. Dysfunction or deletion of PINK1 or Parkin leads to PARIS accumulation, PGC-1 alpha repression, and the degeneration of dopaminergic neurons in PD models (3-5).

    Alternate Names

    FLJ31413; PARIS; Parkin-interacting substrate; parkin-interacting sustrate; Zinc finger protein 746; ZN746; ZNF746

    For Research Use Only. Not for Use in Diagnostic Procedures.
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