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8648
Parkinson's Research Antibody Sampler Kit
Primary Antibodies

Parkinson's Research Antibody Sampler Kit #8648

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Confocal immunofluorescent analysis of MEF wild-type (left) or MEF DJ-1 (-/-) (right) cells using DJ-1 (D29E5) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). (MEF wild-type and MEF DJ-1 (-/-) cells were kindly provided by Dr. Philipp Kahle, University of Tübingen, Germany).

Western blot analysis of extracts from U-87 MG and A172 cells, and mouse brain using LRRK2 (D18E12) Rabbit mAb. 

Western blot analysis of extracts from PC12 cells, fetal rat brain and mouse brain, using Parkin (Prk8) Mouse mAb.

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a cDNA construct expressing full-length human PINK1 (hPINK1, +) using PINK1 (D8G3) Rabbit mAb.

Confocal immunofluorescent analysis of normal rat cerebellum, hippocampus and striatum using α-Synuclein (D37A6) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO* is added and emits light during enzyme catalyzed decomposition.

Western blot analysis of extracts from MEF wild-type, MEF DJ-1 (-/-), HeLa, and C6 cells using DJ-1 (D29E5) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). (MEF wild-type and MEF DJ-1 (-/-) cells were kindly provided by Dr. Philipp Kahle, University of Tübingen, Germany).

Western blot analysis of extracts from HeLa cells, untreated (-) or treated with CCCP (10 μM, 24 hr; +), using PINK1 (D8G3) Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded mouse brain using α-Synuclein (D37A6) XP® Rabbit mAb.

Western blot analysis of extracts from mouse and rat brain using α-Synuclein (D37A6) XP® Rabbit mAb.

To Purchase # 8648T
Product # Size Price
8648T
1 Kit  (5 x 20 µl) $ 380

Product Includes Quantity Applications Reactivity MW(kDa) Isotype
DJ-1 (D29E5) XP® Rabbit mAb 5933 20 µl
  • WB
  • IP
  • IF
H M R Hm Mk 22 Rabbit IgG
LRRK2 (D18E12) Rabbit mAb 13046 20 µl
  • WB
  • IP
H M R 290 Rabbit IgG
Parkin (Prk8) Mouse mAb 4211 20 µl
  • WB
  • IP
H M R 50 Mouse IgG2b
PINK1 (D8G3) Rabbit mAb 6946 20 µl
  • WB
  • IP
H 60, 50 Rabbit IgG
α-Synuclein (D37A6) XP® Rabbit mAb 4179 20 µl
  • WB
  • IP
  • IHC
  • IF
M R 18 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
  • WB
Goat 
Anti-mouse IgG, HRP-linked Antibody 7076 100 µl
  • WB
Horse 

Product Description

The Parkinson's Research Antibody Sampler Kit provides an economical means of detecting target proteins related to Parkinson's disease. The kit contains enough primary and secondary antibody to perform two western blots per primary.

Specificity / Sensitivity

DJ-1 (D29E5) XP® Rabbit mAb, LRRK2 (D18E12) Rabbit mAb, Parkin (Prk8) Mouse mAb, and PINK1 (D8G3) Rabbit mAb recognize endogenous levels of respective target proteins. α-Synuclein (D37A6) XP® Rabbit mAb recognizes endogenous levels of the α isoform of synuclein protein.

Source / Purification

Monoclonal antibodies are produced by immuninzing animals with a recombinant protein specific to the carboxy terminus of Parkin protein, a synthetic peptide corresponding to residues surrounding Lys148 of human DJ-1 protein, a synthetic peptide corresponding to residues surrounding Pro2080 of human LRRK2 protein, a synthetic peptide corresponding to residues surrounding Pro140 of human PINK1 protein, or a synthetic peptide corresponding to residues surrounding Glu105 of mouse α-synuclein protein.

Background

Parkinson’s disease (PD), the second most common neurodegenerative disease after Alzheimer’s, is a progressive movement disorder characterized by rigidity, tremors, and postural instability. The pathological hallmark of PD is progressive loss of dopaminergic neurons in the substantia nigra of the ventral midbrain and the presence of intracellular Lewy bodies in surviving neurons of the brain stem (1). Research studies have shown that various genes and loci (α-synuclein/PARK1 and 4, parkin/PARK2, UCH-L1/PARK5, PINK1/PARK6, DJ-1/PARK7, LRRK2/PARK8, synphilin-1, and NR4A2) are genetically linked to PD (2).

α-Synuclein, a 140 amino acid protein expressed abundantly in the brain, is a major component of aggregates found in Lewy bodies (3). Parkin is involved in protein degradation through the ubiquitin-proteasome pathway, and investigators have shown that mutations in Parkin cause early onset of PD (4). In the case of autosomal recessive juvenile Parkinsonism (AR-JP), deletions have been found on chromosome 6 in the Parkin gene (5). PTEN induced putative kinase 1 (PINK1) is a mitochondrial serine/threonine kinase involved in the normal function and integrity of mitochondria, as well as a reduction of cytochrome c release from mitochondria (6-8). PINK1 phosphorylates Parkin and promotes its translocation to mitochondria (7). Mutations of PINK1 are associated with loss of protective function, mitrochondrial dysfunction, aggregation of α-synuclein, and proteasome dysfunction (6,8). DJ-1 is involved in multiple cellular functions; it has been shown to cooperate with Ras to increase cell transformation, to regulate transcription of the androgen receptor, and may function as an indicator of oxidative stress, while loss-of-function mutations in DJ-1 cause early onset of PD (9-12). Dopamine D2 receptor-mediated functions are greatly impaired in DJ-1 (-/-) mice, resulting in reduced long-term depression (13). Leucine-rich repeat kinase 2 (LRRK2) contains amino-terminal leucine-rich repeats (LRR), a Ras-like small GTP binding protein-like (ROC) domain, an MLK protein kinase domain, and a carboxy-terminal WD40-repeat. At least 20 LRRK2 mutations have been linked to PD (14). The most prevalent mutation, G2019S, causes increased LRRK2 kinase activity, leading to progressive neurite loss and decreased neuronal survival (15).

  1. Fahn, S. (2003) Ann N Y Acad Sci 991, 1-14.
  2. Goldberg, M.S. and Lansbury, P.T. (2000) Nat Cell Biol 2, E115-9.
  3. Borrelli, E. (2005) Neuron 45, 479-81.
  4. Liu, W. et al. (2009) PLoS One 4, e4597.
  5. Moore, D.J. et al. (2005) Annu Rev Neurosci 28, 57-87.
  6. Kim, Y. et al. (2008) Biochem Biophys Res Commun 377, 975-80.
  7. Bonifati, V. et al. (2003) Science 299, 256-9.
  8. Petit, A. et al. (2005) J Biol Chem 280, 34025-32.
  9. Nagakubo, D. et al. (1997) Biochem Biophys Res Commun 231, 509-13.
  10. Mata, I.F. et al. (2006) Trends Neurosci 29, 286-93.
  11. Takahashi, K. et al. (2001) J Biol Chem 276, 37556-63.
  12. MacLeod, D. et al. (2006) Neuron 52, 587-93.
  13. Polymeropoulos, M.H. et al. (1997) Science 276, 2045-7.
  14. Mitsumoto, A. and Nakagawa, Y. (2001) Free Radic Res 35, 885-93.
  15. Goldberg, M.S. et al. (2005) Neuron 45, 489-96.

Pathways & Proteins

Explore pathways + proteins related to this product.

For Research Use Only. Not For Use In Diagnostic Procedures.

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