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  1. Products /
  2. Primary Antibodies /
  3. PARN (P620) Antibody

PARN (P620) Antibody #3899

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  • WB

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 78
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    PARN (P620) Antibody detects endogenous levels of total PARN protein.

    Species Reactivity:

    Human

    The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.

    Species predicted to react based on 100% sequence homology:

    Bovine, Dog

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino acid sequences surrounding Pro620 of human PARN. Antibodies are purified by Protein A and peptide affinity chromatography.

    Background

    Cellular levels of mRNAs are controlled by mRNA stability, the rate of synthesis and the rate of degradation. The presence and length of the poly(A) tail has been associated with mRNA stability (1). Exonucleolytic shortening of the poly(A) tail is the process that initiates the decay of many eukaryotic mRNAs (2). Poly(A)-specific ribonuclease (PARN) is the enzyme responsible for initiation of deadenylation and exonucleolytic shortening of mRNA transcripts. Through an evolutionarily conserved mechanism, PARN also translationally silences selective mRNAs during early embryonic development (3). PARN is constitutively expressed in most mammalian tissues and plays a critical role in the post-transcriptional control of gene expression (4).

    Database Links

    UniProt ID: O95453


    Entrez-Gene Id: 5073


    Limited Uses

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    Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

    For Research Use Only. Not For Use In Diagnostic Procedures.
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