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PDP - Template Name: Antibody Sampler Kit
PDP - Template ID: *******4a3ef3a
  1. Products /
  2. Primary Antibodies /
  3. PBAF Complex Antibody Sampler Kit

PBAF Complex Antibody Sampler Kit #78779

    Product Information

    Kit Usage Information

    Protocols

    Product Description

    The PBAF Complex Antibody Sampler Kit provides an economical means of detecting members of the PBAF chromatin remodeling complex. ARID2, BRD7, and PBRM1/BAF180 are unique members of the PBAF complex, while Brg1 and SMARCC1/BAF155 are shared with BAF and non-canonical BAF complexes. The kit includes enough antibody to perform two western blot experiments with each primary antibody.

    Specificity / Sensitivity

    Each antibody in the PBAF Complex Antibody Sampler Kit detects endogenous levels of its target protein. In addition, Brg1 (D1Q7F) Rabbit mAb does not cross-react with BRM protein.

    Source / Purification

    Monoclonal antibodies are produced by immunizing rabbits with synthetic peptides corresponding to residues surrounding Val891 of human ARID2, Leu1672 of human PBRM1/BAF180, and Gly975 of human SMARCC1/BAF155, recombinant protein specific to the carboxy terminus of human BRD7, and recombinant protein specific to the amino terminus of human Brg1.

    Background

    ATP-dependent chromatin remodeling complexes play an essential role in the regulation of various nuclear processes, such as gene expression, DNA replication, and repair (1,2). The SWI/SNF chromatin remodeling complex consists of more than 10 subunits with a single molecule of the ATPase catalytic subunit BRM or BRG1, but not both. The activities of these two subunits drive the disruption of histone-DNA contacts that lead to changes in accessibility of crucial regulatory elements within chromatin (2-5). The BRM/BRG1 containing SWI/SNF complexes are recruited to target promoters by transcription factors, such as nuclear receptors, p53, RB, and BRCA1 to regulate gene activation, cell growth, the cell cycle, and differentiation processes (1,6-9).

    PBRM1/BAF180, ARID2, and BRD7 are unique members of the SWI/SNF complex PBAF, which binds to kinetochores in mitotic chromatin (10,11). PBAF is involved in nuclear receptor-mediated transcription and retinoic acid driven gene activation (12,13). PBRM1/BAF180 has been shown to be a potent tumor suppressor, as it is the second-most mutated gene in renal carcinomas (14). ARID2 is the targeting subunit of the PBAF complex and critical for complex stability (15). Bromodomain-containing BRD7 interacts with acetylated histones to regulate gene transcription (16,17). SMARCC1/BAF155 is a core subunit of all BAF complexes including PBAF, and is necessary for efficient nucleosome remodeling by BRG1 in vitro (18).

    1. Ho, L. and Crabtree, G.R. (2010) Nature 463, 474-84.
    2. Becker, P.B. and Hörz, W. (2002) Annu Rev Biochem 71, 247-73.
    3. Eberharter, A. and Becker, P.B. (2004) J Cell Sci 117, 3707-11.
    4. Bowman, G.D. (2010) Curr Opin Struct Biol 20, 73-81.
    5. Gangaraju, V.K. and Bartholomew, B. (2007) Mutat Res 618, 3-17.
    6. Lessard, J.A. and Crabtree, G.R. (2010) Annu Rev Cell Dev Biol 26, 503-32.
    7. Morettini, S. et al. (2008) Front Biosci 13, 5522-32.
    8. Wolf, I.M. et al. (2008) J Cell Biochem 104, 1580-6.
    9. Simone, C. (2006) J Cell Physiol 207, 309-14.
    10. Nie, Z. et al. (2000) Mol Cell Biol 20, 8879-88.
    11. Xue, Y. et al. (2000) Proc Natl Acad Sci U S A 97, 13015-20.
    12. Lemon, B. et al. (2001) Nature 414, 924-8.
    13. Wang, Z. et al. (2004) Genes Dev 18, 3106-16.
    14. Varela, I. et al. (2011) Nature 469, 539-42.
    15. Yan, Z. et al. (2005) Genes Dev 19, 1662-7.
    16. Peng, C. et al. (2006) J Cell Biochem 97, 882-92.
    17. Kaeser, M.D. et al. (2008) J Biol Chem 283, 32254-63.
    18. Phelan, M.L. et al. (1999) Mol Cell 3, 247-53.

    Database Links

    UniProt ID: Q9NPI1 , Q92922 , Q68CP9 , P51532 , Q86U86


    Entrez-Gene Id: 29117 , 6599 , 196528 , 6597 , 55193


    Limited Uses

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    For Research Use Only. Not For Use In Diagnostic Procedures.
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