|Brg1 (D1Q7F) Rabbit mAb 49360||20 µl||
||H M R Mk||220||Rabbit IgG|
|BRD7 (D9K2T) Rabbit mAb 15125||20 µl||
|PBRM1/BAF180 (E9X2Z) Rabbit mAb 89123||20 µl||
||H M R Mk||205||Rabbit IgG|
|ARID2 (D8D8U) Rabbit mAb 82342||20 µl||
|SMARCC1/BAF155 (D7F8S) Rabbit mAb 11956||20 µl||
||H M R Mk||155||Rabbit IgG|
|Anti-rabbit IgG, HRP-linked Antibody 7074||100 µl||
Monoclonal antibodies are produced by immunizing rabbits with synthetic peptides corresponding to residues surrounding Val891 of human ARID2, Leu1672 of human PBRM1/BAF180, and Gly975 of human SMARCC1/BAF155, recombinant protein specific to the carboxy terminus of human BRD7, and recombinant protein specific to the amino terminus of human Brg1.
ATP-dependent chromatin remodeling complexes play an essential role in the regulation of various nuclear processes, such as gene expression, DNA replication, and repair (1,2). The SWI/SNF chromatin remodeling complex consists of more than 10 subunits with a single molecule of the ATPase catalytic subunit BRM or BRG1, but not both. The activities of these two subunits drive the disruption of histone-DNA contacts that lead to changes in accessibility of crucial regulatory elements within chromatin (2-5). The BRM/BRG1 containing SWI/SNF complexes are recruited to target promoters by transcription factors, such as nuclear receptors, p53, RB, and BRCA1 to regulate gene activation, cell growth, the cell cycle, and differentiation processes (1,6-9).
PBRM1/BAF180, ARID2, and BRD7 are unique members of the SWI/SNF complex PBAF, which binds to kinetochores in mitotic chromatin (10,11). PBAF is involved in nuclear receptor-mediated transcription and retinoic acid driven gene activation (12,13). PBRM1/BAF180 has been shown to be a potent tumor suppressor, as it is the second-most mutated gene in renal carcinomas (14). ARID2 is the targeting subunit of the PBAF complex and critical for complex stability (15). Bromodomain-containing BRD7 interacts with acetylated histones to regulate gene transcription (16,17). SMARCC1/BAF155 is a core subunit of all BAF complexes including PBAF, and is necessary for efficient nucleosome remodeling by BRG1 in vitro (18).
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