PCAF (C14G9) Rabbit mAb #3378
- WB
- IP
- ChIP
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 93 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- ChIP-Chromatin Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
For optimal ChIP and ChIP-seq results, use 20 μl of antibody and 10 μg of chromatin (approximately 4 x 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
| Chromatin IP | 1:25 |
| Chromatin IP-seq | 1:25 |
Storage
Protocol
Specificity / Sensitivity
PCAF (C14G9) Rabbit mAb detects endogenous levels of total PCAF protein. The antibody does not cross-react with the related GCN5L2 protein.
Species Reactivity:
Human, Mouse, Rat, Monkey
The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.
Species predicted to react based on 100% sequence homology:
Bovine, Horse
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the amino terminus of human PCAF protein.
Background
p300/CBP-associated factor (PCAF), also known as lysine acetyl-transferase 2B (KAT2B), is a transcriptional adaptor protein and histone acetyl-transferase (HAT) that functions as the catalytic subunit of the PCAF transcriptional co-activator complex (1). PCAF is 73% homologous to GCN5L2, another HAT protein found in similar complexes (1,2). Like GCN5L2, PCAF acetylates histone H3 on Lys14 and histone H4 on Lys8, both of which contribute to gene activation by modulating chromatin structure and recruiting additional co-activator proteins that contain acetyl-lysine binding bromo-domains (3). PCAF also acetylates non-histone proteins including transcriptional activators (p53, E2F1, MyoD), general transcription factors (TFIIEβ and TFIIF) and architectural DNA binding proteins (HMGA1 and HMG17) (4-10). Acetylation of these proteins regulates their nuclear localization, protein stability, DNA binding, and co-activator association.
- Nagy, Z. and Tora, L. (2007) Oncogene 26, 5341-57.
- Yang, X.J. et al. (1996) Nature 382, 319-24.
- Schiltz, R.L. et al. (1999) J Biol Chem 274, 1189-92.
- Bannister, A.J. and Miska, E.A. (2000) Cell Mol Life Sci 57, 1184-92.
- Liu, L. et al. (1999) Mol Cell Biol 19, 1202-9.
- Martínez-Balbás, M.A. et al. (2000) EMBO J 19, 662-71.
- Sartorelli, V. et al. (1999) Mol Cell 4, 725-34.
- Imhof, A. et al. (1997) Curr Biol 7, 689-92.
- Munshi, N. et al. (1998) Mol Cell 2, 457-67.
- Herrera, J.E. et al. (1999) Mol Cell Biol 19, 3466-73.
Limited Uses
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