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71395
PCNA (D3H8P) XP® Rabbit mAb (BSA and Azide Free)
Primary Antibodies
Monoclonal Antibody

PCNA (D3H8P) XP® Rabbit mAb (BSA and Azide Free) #71395

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  1. WB
  2. IHC
  3. IF
  4. F
Western Blotting Image 1: PCNA (D3H8P) XP® Rabbit mAb (BSA and Azide Free)
Western blot analysis of extracts from various cell lines using PCNA (D3H8P) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 1: PCNA (D3H8P) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using PCNA (D3H8P) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 2: PCNA (D3H8P) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using PCNA (D3H8P) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunofluorescence Image 1: PCNA (D3H8P) XP® Rabbit mAb (BSA and Azide Free)
Confocal immunofluorescent analysis of fixed frozen rat small intestine using PCNA (D3H8P) XP® Rabbit mAb (green), β-Catenin (L54E2) Mouse mAb (IF Preferred) #2677 (red), and DRAQ5® #4084 (fluorescent DNA dye) (blue). Data were generated using the standard formulation of this product.
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Immunofluorescence Image 1: PCNA (D3H8P) XP® Rabbit mAb (BSA and Azide Free)
Confocal immunofluorescent analysis of HeLa cells using PCNA (D3H8P) XP® Rabbit mAb (green) and β-Actin (8H10D10) Mouse mAb #3700 (red). Data were generated using the standard formulation of this product.
Flow Cytometry Image 1: PCNA (D3H8P) XP® Rabbit mAb (BSA and Azide Free)
Flow cytometric analysis of Jurkat cells using PCNA (D3H8P) XP® Rabbit mAb and Propidium Iodide (PI)/RNase Staining Solution #4087 to measure DNA content. Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody. Data were generated using the standard formulation of this product.
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To Purchase # 71395
Cat. # Size Qty. Price
71395SF
100 µg
$ 746

Supporting Data

REACTIVITY H M R Mk
SENSITIVITY Endogenous
MW (kDa) 36
Source/Isotype Rabbit IgG

Application Key:

  • WB-Western Blot
  • IP-Immunoprecipitation
  • IHC-Immunohistochemistry
  • ChIP-Chromatin Immunoprecipitation
  • C&R-CUT&RUN
  • C&T-CUT&Tag
  • DB-Dot Blot
  • eCLIP-eCLIP
  • IF-Immunofluorescence
  • F-Flow Cytometry

Species Cross-Reactivity Key:

  • H-Human
  • M-Mouse
  • R-Rat
  • Hm-Hamster
  • Mk-Monkey
  • Vir-Virus
  • Mi-Mink
  • C-Chicken
  • Dm-D. melanogaster
  • X-Xenopus
  • Z-Zebrafish
  • B-Bovine
  • Dg-Dog
  • Pg-Pig
  • Sc-S. cerevisiae
  • Ce-C. elegans
  • Hr-Horse
  • All-All Species Expected

Product Usage Information

This product is the carrier free version of product #13110. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. Optimal dilutions/concentrations should be determined by the end user.

Formulation

Supplied in 1X PBS, BSA and Azide Free.

For standard formulation of this product see product #13110.

Storage

Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.

Specificity / Sensitivity

PCNA (D3H8P) XP® Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total PCNA protein.

Species Reactivity:

Human, Mouse, Rat, Monkey

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human PCNA protein.

Background

Proliferating cell nuclear antigen (PCNA) is a member of the DNA sliding clamp family of proteins that assist in DNA replication (1). Crystal structure data suggests that a PCNA homotrimer ring can encircle and slide along the DNA double helix (2). Multiple proteins involved in DNA replication, DNA repair, and cell cycle control bind to PCNA rather than directly associating with DNA, thus facilitating fast processing of DNA (reviewed in 3). PCNA protein expression is a well-accepted marker of proliferation.
  1. Kelman, Z. and O'Donnell, M. (1995) Nucleic Acids Res 23, 3613-20.
  2. Krishna, T.S. et al. (1994) Cell 79, 1233-43.
  3. Maga, G. and Hubscher, U. (2003) J Cell Sci 116, 3051-60.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
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