PCNA (PC10) Mouse mAb #2586
- WB
- IP
- IHC
- IF
- F
Supporting Data
| REACTIVITY | H M R Mk B Pg |
| SENSITIVITY | Endogenous |
| MW (kDa) | 36 |
| Source/Isotype | Mouse IgG2a |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IHC-Immunohistochemistry
- IF-Immunofluorescence
- F-Flow Cytometry
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
- B-Bovine
- Pg-Pig
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:2000 |
| Immunoprecipitation | 1:100 |
| Immunohistochemistry (Paraffin) | 1:8000 - 1:32000 |
| Immunofluorescence (Immunocytochemistry) | 1:1600 - 1:3200 |
| Flow Cytometry (Fixed/Permeabilized) | 1:400 |
Storage
For a carrier free (BSA and azide free) version of this product see product #85118.
Protocol
Specificity / Sensitivity
PCNA (PC10) Mouse mAb detects endogenous levels of PCNA protein.
Species Reactivity:
Human, Mouse, Rat, Monkey, Bovine, Pig
Source / Purification
Monoclonal antibody is produced by immunizing animals with a recombinant Protein A-PCNA fusion protein obtained from pC2T.
Background
Proliferating cell nuclear antigen (PCNA) is a member of the DNA sliding clamp family of proteins that assist in DNA replication (1). Crystal structure data suggests that a PCNA homotrimer ring can encircle and slide along the DNA double helix (2). Multiple proteins involved in DNA replication, DNA repair, and cell cycle control bind to PCNA rather than directly associating with DNA, thus facilitating fast processing of DNA (reviewed in 3). PCNA protein expression is a well-accepted marker of proliferation.
Limited Uses
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