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  2. Primary Antibodies /
  3. PDAP1 Antibody

PDAP1 Antibody #4300

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  • WB
  • IP

    Supporting Data

    REACTIVITY H M R Mk
    SENSITIVITY Endogenous
    MW (kDa) 25
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    PDAP1 Antibody detects endogenous levels of total PDAP1 protein. Nonspecific bands are seen above 60 KDa.

    Species Reactivity:

    Human, Mouse, Rat, Monkey

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the center of human PDAP1 protein. Antibodies were purified by protein A and peptide affinity chromatography.

    Background

    Platelet derived growth factor (PDGF) proteins function as dimeric isoforms (i.e., PDGF AA, PDGF AB, PDGF BB, PDGF CC and PDGF DD) that bind receptor tyrosine kinases and activate cytoplasmic SH2 domain-containing proteins to control multiple signaling pathways that regulate angiogenesis, cell growth, actin reorganization, migration and differentiation (1). PDGFA-associated protein 1 (PDAP1) was originally identified as a novel, PDGF-associated protein found in a rat retinal tumor cell line (2). While copurified with PDGFA, PDAP1 interacts with PDGFB at a slightly higher affinity than with PDGFA (2). Although the exact function of PDAP1 is unclear, it has been shown to both increase PDGFA-induced incorporation of [3H]thymidine in Swiss 3T3 cells and decrease PDGFB growth factor activity (2). Ubiquitously expressed PDAP1 is highly conserved among species (2,3) and is phosphorylated in vitro by several kinases, including PKC, PKA, CKI and CKII. Among this group, CKII seems to be the major kinase that phosphorylates PDAP1 in intact cells (3).

    Database Links

    UniProt ID: Q13442


    Entrez-Gene Id: 11333


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    For Research Use Only. Not For Use In Diagnostic Procedures.
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