Immunohistochemical analysis of paraffin-embedded human ductal breast carcinoma using PDI (E7O2R) Mouse mAb.
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using PDI (E7O2R) Mouse mAb (left) compared to concentration-matched Mouse (G3A1) mAb IgG1 Isotype Control #5415 (right).
Immunohistochemical analysis of paraffin-embedded normal human kidney using PDI (E7O2R) Mouse mAb.
Immunohistochemical analysis of paraffin-embedded normal human liver using PDI (E7O2R) Mouse mAb.
Immunohistochemical analysis of paraffin-embedded human squamous cell lung carcinoma using PDI (E7O2R) Mouse mAb.
Immunohistochemical analysis of paraffin-embedded human non-small cell lung carcinoma using PDI (E7O2R) Mouse mAb.
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using PDI (E7O2R) Mouse mAb.
Immunohistochemical analysis of paraffin-embedded normal human spleen using PDI (E7O2R) Mouse mAb.
Confocal immunofluorescent analysis of HeLa cells using PDI (E7O2R) Mouse mAb (green) and β-Actin (13E5) Rabbit mAb #4970 (red). Samples were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).
|REACTIVITY||H M R Mk|
|Source/Isotype||Mouse IgG1 kappa|
|Immunohistochemistry (Paraffin)||1:400 - 1:1600|
|Immunofluorescence (Immunocytochemistry)||1:100 - 1:400|
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.
NOTE: Do not allow slides to dry at any time during this procedure.
For Citrate: Heat slides in a microwave submersed in 1X citrate unmasking solution until boiling is initiated; follow with 10 min at a sub-boiling temperature (95°-98°C). Cool slides on bench top for 30 min.
|SignalStain® Boost IHC Detection Reagent (HRP, Mouse) #8125||SignalStain® Boost IHC Detection Reagent (AP, Mouse) #31926|
|SignalStain® DAB Substrate Kit #8059||SignalStain® Vibrant Red Alkaline Phosphatase Substrate Kit #76713|
|SignalStain® Vivid Purple Peroxidase Substrate Kit #96632|
NOTE: Use of detection reagents other than those specified in this protocol may require further optimization of the primary antibody to account for the different sensitivities of the detection reagents.
posted February 2010
revised June 2020
Protocol Id: 280
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalently purified water.
Recommended Fluorochrome-conjugated Anti-Mouse secondary antibodies:
NOTE: Cells should be grown, treated, fixed and stained directly in multiwell plates, chamber slides or on coverslips.
NOTE: All subsequent incubations should be carried out at room temperature unless otherwise noted in a humid light-tight box or covered dish/plate to prevent drying and fluorochrome fading.
posted November 2006
revised December 2010
Protocol Id: 146
PDI (E7O2R) Mouse mAb recognizes endogenous levels of total PDI protein.
Human, Mouse, Rat, Monkey
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu332 of human PDI protein.
During their synthesis, secretory proteins translocate into the endoplasmic reticulum (ER) where they are post-translationally modified and properly folded. To reach their native conformation, many secretory proteins require the formation of intra- or inter-molecular disulfide bonds (1). This process is called oxidative protein folding. Protein disulfide isomerase (PDI) catalyzes the formation and isomerization of these disulfide bonds (2). Studies on mechanisms of oxidative folding suggest that molecular oxygen oxidizes the ER-protein Ero1, which in turn oxidizes PDI through disulfide exchange (3). This event is then followed by PDI-catalyzed disulfide bond formation in folding proteins (3).
Explore pathways + proteins related to this product.