Render Target: STATIC
Render Timestamp: 2024-12-06T12:21:08.536Z
Commit: 224419269841c11382c4555dbee545259bf6c379
XML generation date: 2024-08-15 10:49:08.455
Product last modified at: 2024-09-09T13:45:09.470Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

Perilipin-1 (K117) Antibody #3467

Filter:
  • WB
  • IF

    Supporting Data

    REACTIVITY H M
    SENSITIVITY Endogenous
    MW (kDa) 62
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunofluorescence (Immunocytochemistry) 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Perilipin-1 (K117) Antibody detects endogenous levels of total perilipin-1 protein.

    Species Reactivity:

    Human, Mouse

    The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.

    Species predicted to react based on 100% sequence homology:

    Pig

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence around Lys117 of human perilipin-1. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    Triacylglycerol is stored in lipid droplets as a primary energy reserve. Perilipin is localized at the periphery of lipid droplets and serves as a protective coating against lipases (1-3). Evidence suggests that PKA regulates lipolysis by phosphorylating perilipin (1,2,4,5). Phosphorylation of perilipin results in the conformational change that exposes lipid droplets to endogenous lipases, such as hormone-sensitive lipases (2). Hence, perilipin plays a pivotal role in lipid storage (2,5).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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