Render Target: STATIC
Render Timestamp: 2024-11-01T09:45:26.578Z
Commit: 23cb9f61fe67e1e9093fd644a533c4ff516a6463
XML generation date: 2024-08-01 15:28:13.388
Product last modified at: 2024-10-11T12:00:17.633Z
1% for the planet logo
PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

PGAM5 (Long Isoform) Antibody #24584

Filter:
  • WB

    Supporting Data

    REACTIVITY H M R Mk
    SENSITIVITY Endogenous
    MW (kDa) 28, 30
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    PGAM5 (Long Isoform) Antibody recognizes endogenous levels of total PGAM5 long isoform protein. This antibody specifically recognizes the PGAM5 long isoform (UniProt #Q96HS1-1) and does not recognize the PGAM5 short isoform (UniProt #Q96HS1-2).

    Species Reactivity:

    Human, Mouse, Rat, Monkey

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human PGAM5 long isoform (UniProt #Q96HS1-1). Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    Phosphoglycerate mutase family 5 (PGAM5) is a mitochondrial serine/threonine protein phosphatase (1,2). PGAM5 is required for necrosis induced by TNF-α or reactive oxygen species (2). In addition, PGAM5 dephosphorylates FUNDC1 to induce mitophagy under mitochondrial stresses (3). PGAM5 is also essential for RIPK1-mediated mitophagy activation during extracellular matrix (ECM) detachment of cancer cells (4). Furthermore, PGAM5 is cleaved by the protease presenilin-associated rhomboid-like protein (PARL) in response to mitochondrial stresses (such as induced by carbonyl cyanide m-chlorophenylhydrazone (CCCP) treatment). The cleaved PGAM5, released from the mitochondrial membrane, dephosphorylates β-catenin in the cytosol leading to its stabilization. Increased levels of β-catenin stimulate mitochondrial biogenesis (5).
    For Research Use Only. Not For Use In Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    All other trademarks are the property of their respective owners. Visit our Trademark Information page.