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PHF5A (F1P9L) Rabbit Monoclonal Antibody #89567

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  • WB
  • IP

    Product Specifications

    REACTIVITY H M R Mk
    SENSITIVITY Endogenous
    MW (kDa) 12
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    PHF5A (F1P9L) Rabbit Monoclonal Antibody recognizes endogenous levels of total PHF5A protein.

    Species Reactivity:

    Human, Mouse, Rat, Monkey

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly106 of human PHF5A protein.

    Background

    PHF5A, also known as SAP14B, is a highly conserved nuclear PHD domain-containing protein that is a critical component of the U2 small nuclear ribonucleoprotein (U2 snRNP) and plays an important role in the splicing of pre-mRNA that involves the removal of introns and the joining of exons to form mature mRNA (1-3). The assembly and proper recognition of splice sites are driven by sequences at the pre-mRNA intron-exon splice sites. The 5’ splice donor site is recognized by the U1 snRNP complex, while U2 snRNP binds to the 3’ splice site (branch point), ensuring the anchoring of the spliceosome machinery at the splice sites (4,5). Mutations in PHF5A are lethal, as the protein serves an essential role in stabilizing the SF3b spliceosomal complex and linking it to histones to promote alternative splicing (6). PHF5A is overexpressed in many types of cancers, where it promotes growth factors and suppresses apoptotic signals (6-8). PHF5A has also been shown to be a critical regulator in pluripotency as well as neuronal development (9,10).

    Alternate Names

    bK223H9.2; INI; MGC1346; PHD finger protein 5A; PHD finger-like domain protein 5A; PHD finger-like domain-containing protein 5A; PHD-finger 5a; PHF5A; Rds3; SAP14b; SF3b14b; SF3B7; splicing factor 3B associated 14 kDa protein; Splicing factor 3B-associated 14 kDa protein; splicing factor 3b, subunit 7

    For Research Use Only. Not for Use in Diagnostic Procedures.
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