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PHF6 Antibody
Primary Antibodies
Polyclonal Antibody

PHF6 Antibody #44438

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  1. WB
Western Blotting Image 1 - PHF6 Antibody

Western blot analysis of extracts from various cell lines using PHF6 Antibody.

To Purchase # 44438S
Product # Size Price
100 µl $ 268

Supporting Data

MW (kDa) 45 (human/monkey), 41 (mouse/rat)

Application Key:

  • W-Western
  • IP-Immunoprecipitation
  • IHC-Immunohistochemistry
  • ChIP-Chromatin Immunoprecipitation
  • IF-Immunofluorescence
  • F-Flow Cytometry
  • E-P-ELISA-Peptide

Species Cross-Reactivity Key:

  • H-Human
  • M-Mouse
  • R-Rat
  • Hm-Hamster
  • Mk-Monkey
  • Mi-Mink
  • C-Chicken
  • Dm-D. melanogaster
  • X-Xenopus
  • Z-Zebrafish
  • B-Bovine
  • Dg-Dog
  • Pg-Pig
  • Sc-S. cerevisiae
  • Ce-C. elegans
  • Hr-Horse
  • All-All Species Expected

Product Usage Information

Application Dilution
Western Blotting 1:1000


Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.



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Western Blotting Protocol

For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.

NOTE: Please refer to primary antibody product webpage for recommended antibody dilution.

A. Solutions and Reagents

From sample preparation to detection, the reagents you need for your Western Blot are now in one convenient kit: #12957 Western Blotting Application Solutions Kit

NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.

  1. 20X Phosphate Buffered Saline (PBS): (#9808) To prepare 1 L 1X PBS: add 50 ml 20X PBS to 950 ml dH2O, mix.
  2. 10X Tris Buffered Saline (TBS): (#12498) To prepare 1 L 1X TBS: add 100 ml 10X to 900 ml dH2O, mix.
  3. 1X SDS Sample Buffer: Blue Loading Pack (#7722) or Red Loading Pack (#7723) Prepare fresh 3X reducing loading buffer by adding 1/10 volume 30X DTT to 1 volume of 3X SDS loading buffer. Dilute to 1X with dH2O.
  4. 10X Tris-Glycine SDS Running Buffer: (#4050) To prepare 1 L 1X running buffer: add 100 ml 10X running buffer to 900 ml dH2O, mix.
  5. 10X Tris-Glycine Transfer Buffer: (#12539) To prepare 1 L 1X Transfer Buffer: add 100 ml 10X Transfer Buffer to 200 ml methanol + 700 ml dH2O, mix.
  6. 10X Tris Buffered Saline with Tween® 20 (TBST): (#9997) To prepare 1 L 1X TBST: add 100 ml 10X TBST to 900 ml dH2O, mix.
  7. Nonfat Dry Milk: (#9999).
  8. Blocking Buffer: 1X TBST with 5% w/v nonfat dry milk; for 150 ml, add 7.5 g nonfat dry milk to 150 ml 1X TBST and mix well.
  9. Wash Buffer: (#9997) 1X TBST.
  10. Bovine Serum Albumin (BSA): (#9998).
  11. Primary Antibody Dilution Buffer: 1X TBST with 5% BSA; for 20 ml, add 1.0 g BSA to 20 ml 1X TBST and mix well.
  12. Biotinylated Protein Ladder Detection Pack: (#7727).
  13. Prestained Protein Marker, Broad Range (11-190 kDa): (#13953).
  14. Blotting Membrane and Paper: (#12369) This protocol has been optimized for nitrocellulose membranes. Pore size 0.2 µm is generally recommended.
  15. Secondary Antibody Conjugated to HRP: Anti-rabbit IgG, HRP-linked Antibody (#7074).
  16. Detection Reagent: SignalFire™ ECL Reagent (#6883).

B. Protein Blotting

A general protocol for sample preparation.

  1. Treat cells by adding fresh media containing regulator for desired time.
  2. Aspirate media from cultures; wash cells with 1X PBS; aspirate.
  3. Lyse cells by adding 1X SDS sample buffer (100 µl per well of 6-well plate or 500 µl for a 10 cm diameter plate). Immediately scrape the cells off the plate and transfer the extract to a microcentrifuge tube. Keep on ice.
  4. Sonicate for 10–15 sec to complete cell lysis and shear DNA (to reduce sample viscosity).
  5. Heat a 20 µl sample to 95–100°C for 5 min; cool on ice.
  6. Microcentrifuge for 5 min.
  7. Load 20 µl onto SDS-PAGE gel (10 cm x 10 cm).

    NOTE: Loading of prestained molecular weight markers (#13953, 5 µl/lane) to verify electrotransfer and biotinylated protein ladder (#7727, 10 µl/lane) to determine molecular weights are recommended.

  8. Electrotransfer to nitrocellulose membrane (#12369).

C. Membrane Blocking and Antibody Incubations

NOTE: Volumes are for 10 cm x 10 cm (100 cm2) of membrane; for different sized membranes, adjust volumes accordingly.

I. Membrane Blocking

  1. (Optional) After transfer, wash nitrocellulose membrane with 25 ml TBS for 5 min at room temperature.
  2. Incubate membrane in 25 ml of blocking buffer for 1 hr at room temperature.
  3. Wash three times for 5 min each with 15 ml of TBST.

II. Primary Antibody Incubation

  1. Incubate membrane and primary antibody (at the appropriate dilution and diluent as recommended in the product webpage) in 10 ml primary antibody dilution buffer with gentle agitation overnight at 4°C.
  2. Wash three times for 5 min each with 15 ml of TBST.
  3. Incubate membrane with Anti-rabbit IgG, HRP-linked Antibody (#7074 at 1:2000) and anti-biotin, HRP-linked Antibody (#7075 at 1:1000–1:3000) to detect biotinylated protein markers in 10 ml of blocking buffer with gentle agitation for 1 hr at room temperature.
  4. Wash three times for 5 min each with 15 ml of TBST.
  5. Proceed with detection (Section D).

D. Detection of Proteins

Directions for Use:

  1. Wash membrane-bound HRP (antibody conjugate) three times for 5 minutes in TBST.
  2. Prepare 1X SignalFire™ ECL Reagent (#6883) by diluting one part 2X Reagent A and one part 2X Reagent B (e.g. for 10 ml, add 5 ml Reagent A and 5 ml Reagent B). Mix well.
  3. Incubate substrate with membrane for 1 minute, remove excess solution (membrane remains wet), wrap in plastic and expose to X-ray film.

* Avoid repeated exposure to skin.

posted June 2005

revised June 2020

Protocol Id: 10

Specificity / Sensitivity

PHF6 Antibody recognizes endogenous levels of total PHF6 protein.

Species Reactivity:

Human, Mouse, Rat, Monkey

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly63 of human PHF6 protein. Antibodies are purified by protein A and peptide affinity chromatography.


Plant homeodomain-like finger 6 (PHF6) is a 41 kDa transcriptional repressor that was first identified as a mutated gene in Börjeson-Forssman-Lehmann syndrome (BFLS), an X-linked intellectual disability disorder (1,2). Somatic loss-of-function mutations in the PHF6 gene have also been linked to T-cell acute lymphoblastic leukemia (T-ALL) and acute myeloid leukemia (AML) (3-5). Structurally, PHF6 contains two nuclear localization sequences, one nucleolar localization sequence, and two plant homeodomain (PHD)-like zinc fingers (6,7). Unlike other PHD proteins, the PHD domains of PHF6 are considered to be imperfect and have not been shown to directly bind to histones; however, the isolated second PHD domain (PHD2) has been shown to bind dsDNA directly (7). A more recent study finds that PHF6 interacts with histones via protein-protein interactions, and that this association is independent of DNA and enriched in the presence of the activating marks H3K27ac and H3K4me3 (8). PHF6 interacts with PAF1 and other subunits of the PAF1 transcription elongation complex, and knockdown of either PHF6 or PAF1 adversely affects proper neuronal positioning and migration in mouse cerebral cortex (9). PHF6 has also been shown to associate with members of the nucleosome remodeling and deacetylation (NuRD) chromatin remodeling complex, including CHD4, HDAC1, and RBBP4, where it is likely involved in transcriptional repression of developmental genes (10). PHF6 plays a critical role in regulating hematopoiesis, particularly by regulating chromatin accessibility to lineage-specific transcription factors. Studies suggest that PHF6 promotes B-cell lineage differentiation through the expression of B-cell specific genes, while simultaneously suppressing T-cell lineage differentiation (8). Indeed, a CRISPR-Cas9 knockout study shows that PHF6 is required for growth of B-ALL cells, while mice transplanted with PHF6-deficient B-ALL cells develop T-ALL phenotypes (8).

  1. Lower, K.M. et al. (2002) Nat Genet 32, 661-5.
  2. Gedeon, A.K. et al. (1996) Am J Med Genet 64, 63-8.
  3. Van Vlierberghe, P. et al. (2010) Nat Genet 42, 338-42.
  4. de Rooij, J.D. et al. (2016) Br J Haematol 175, 967-71.
  5. Van Vlierberghe, P. et al. (2011) Leukemia 25, 130-4.
  6. Jahani-Asl, A. et al. (2016) Neurobiol Dis 96, 227-35.
  7. Liu, Z. et al. (2014) J Biol Chem 289, 10069-83.
  8. Soto-Feliciano, Y.M. et al. (2017) Genes Dev 31, 973-89.
  9. Zhang, C. et al. (2013) Neuron 78, 986-93.
  10. Todd, M.A. and Picketts, D.J. (2012) J Proteome Res 11, 4326-37.

Pathways & Proteins

Explore pathways + proteins related to this product.

For Research Use Only. Not For Use In Diagnostic Procedures.
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