Phospho-Ack1 (Tyr857/858) Antibody #3137
- WB
Inquiry Info. # 3137
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Product Specifications
| REACTIVITY | H M |
| SENSITIVITY | Transfected Only |
| MW (kDa) | 135 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
Phosphorylation of Ack1 on Tyr857 and Tyr858 on Ack1 was identified at Cell Signaling Technology (CST) using PhosphoScan®, CST's LC-MS/MS platform for phosphorylation site discovery as well as other publications using MS technology (5,6). Phosphorylation of Ack1 at Tyr857/858 was observed in select carcinoma cell lines and in tumors.
- Galisteo, M.L. et al. (2006) Proc. Natl. Acad. Sci. USA 103, 9796-9801.
- Yokoyama, N. and Miller, W.T. (2003) J. Biol. Chem. 278, 47713-47723.
- Yang, W. and Cerione, R.A. (1997) J. Biol. Chem. 272, 24819-24824.
- Yang, W. et al. (2001) J. Biol. Chem. 276, 43987-43993.
- Mahajan, N.P. et al. (2005) Cancer Res. 65, 10514-10523.
- Zhang, Y. et al. (2005) Mol. Cell Proteomics 4, 1240-1250.
- Salomon, A.R. et al. (2003) Proc. Natl. Acad. Sci. USA 100, 443-448.
Alternate Names
ACK; ACK-1; ACK1; Activated CDC42 kinase 1; activated Cdc42-associated kinase 1; activated p21cdc42Hs kinase; FLJ44758; FLJ45547; p21cdc42Hs; TNK2; tyrosine kinase non receptor 2; Tyrosine kinase non-receptor protein 2; tyrosine kinase, non-receptor, 2
Limited Uses
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