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Phospho-Axl (Tyr698)/Mer (Tyr749)/Tyro3 (Tyr681) (D6M4W) Rabbit Monoclonal Antibody #44463

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  • WB
  • IP

    Product Specifications

    REACTIVITY H
    SENSITIVITY Transfected Only
    MW (kDa) 110-130, 125-140, 180-200
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Phospho-Axl (Tyr698)/Mer (Tyr749)/Tyro3 (Tyr681) (D6M4W) Rabbit Monoclonal Antibody detects immunoprecipitated or transfected levels of Axl/Mer/Tyro3 protein phosphorylated at Axl (Tyr698)/Mer (Tyr749)/Tyro3 (Tyr681). This antibody may cross-react with other phosphorylated tyrosine proteins.

    Species Reactivity:

    Human

    The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.

    Species predicted to react based on 100% sequence homology:

    Mouse, Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with synthetic phospho-peptides corresponding to residues surrounding Tyr698/Tyr749 of human Axl/Mer protein.

    Background

    Axl, Mer and Tyro3 are three members of the TAM family receptor tyrosine kinase that share a common NCAM (neural adhesion molecule)-related extracellular domain and a conserved intracellular tyrosine kinase domain. These receptors bind common homologous vitamin K dependent protein GAS6 and protein S to activate downstream signaling pathways (1). TAM family receptors are involved in the development of immune, nervous, vascular and reproductive systems, autoimmune disease, cancer drug resistance and tumor immunity response (2-5). Axl (Tyr698), Axl (Tyr702), Mer Tyr(749) and Tyro3 (Tyr681) are conserved autophosphorylation sites located in the activation loop of the respective tyrosine kinase domains. Phosphorylation at these sites is required for full kinase activation of each of the corresponding receptors (6,7).

    Alternate Names

    ARK; AXL; AXL oncogene; AXL receptor tyrosine kinase; AXL transforming sequence/gene; Brt; BYK; c-Eyk; c-mer; c-mer proto-oncogene tyrosine kinase; DTK; Etk-2; FLJ16467; JTK11; MER; MER proto-oncogene, tyrosine kinase; MER receptor tyrosine kinase; MERTK; MGC133349; oncogene AXL; Proto-oncogene c-Mer; Receptor tyrosine kinase MerTK; Rek; RP38; RSE; SKY; STK kinase; Tif; Tyro12; TYRO3; TYRO3 protein tyrosine kinase; Tyro3 protein tyrosine kinase (sea-related receptor tyrosine kinase); Tyro7; Tyrosine-protein kinase byk; Tyrosine-protein kinase DTK; Tyrosine-protein kinase Mer; Tyrosine-protein kinase receptor TYRO3; Tyrosine-protein kinase receptor UFO; Tyrosine-protein kinase RSE; Tyrosine-protein kinase SKY; Tyrosine-protein kinase TIF; UFO

    For Research Use Only. Not for Use in Diagnostic Procedures.
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