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9111
Phospho-cdc2 (Tyr15) Antibody

Phospho-cdc2 (Tyr15) Antibody #9111

Western Blotting

Western blot analysis of extracts from HeLa cells, either untreated (-) or treated with hydroxyurea (4 mM, 20 hr; +) using Phospho-cdc2 (Tyr15) Antibody (upper) or cdc2 (E1Z6R) Rabbit mAb #28439 (lower).

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Application Dilutions
Western Blotting 1:1000
Immunoprecipitation 1:50
Storage:

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Phospho-cdc2 (Tyr15) Antibody detects endogenous levels of cdc2, CDK2 and CDK5 only when phosphorylated at tyrosine 15. Based on sequence similarity, the antibody may also cross-react with CDK3. The antibody does not cross-react with CDK4, CDK6 or CDK7. It does detect the yeast orthologue of cdc2 (cdc28) when phosphorylated at tyrosine 19.

Species Reactivity:

Human, Mouse, Rat, Monkey, D. melanogaster, Xenopus, S. cerevisiae

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr15 of human cdc2. Antibodies are purified by protein A and peptide affinity chromatography.

The entry of eukaryotic cells into mitosis is regulated by cdc2 kinase activation, a process controlled at several steps including cyclin binding and phosphorylation of cdc2 at Thr161 (1). However, the critical regulatory step in activating cdc2 during progression into mitosis appears to be dephosphorylation of cdc2 at Thr14 and Tyr15 (2). Phosphorylation at Thr14 and Tyr15, resulting in inhibition of cdc2, can be carried out by Wee1 and Myt1 protein kinases (3,4). The cdc25 phosphatase may be responsible for removal of phosphates at Thr14 and Tyr15 and subsequent activation of cdc2 (1,5).

  1. Atherton-Fessler, S. et al. (1994) Mol Biol Cell 5, 989-1001.
  2. Norbury, C. et al. (1991) EMBO J 10, 3321-9.
  3. McGowan, C.H. and Russell, P. (1993) EMBO J 12, 75-85.
  4. Wells, N.J. et al. (1999) J Cell Sci 112 ( Pt 19), 3361-71.
  5. Hunter, T. (1995) Cell 80, 225-36.
Entrez-Gene Id
983
Swiss-Prot Acc.
P06493
For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

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Technical Support and Customer Service Closing Early Today

US Customer Service and Technical Support will be closing at 3:00 PM EST today. Any US orders placed today will ship on Tuesday, May 29.

To Purchase # 9111S

View sizes
Product # Size Price
9111T
20 µl (2 western blots) $ 120.0
9111S
100 µl (10 western blots) $ 297.0
9111L
300 µl (30 western blots) $ 702.0