Phospho-cdc25C (Thr48) Antibody #9527
- WB
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 75 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
Full activation of cdc25C involves phosphorylation at more than 12 different sites by cdc2/cyclin B and Polo-like kinase, and the activity of Pin1, a peptidyl-prolyl isomerase (PPI) (6,7). Pin1 contains a WW domain that binds phospho-Ser/Thr-Pro sites and a catalytic PPI region that induces a cis/trans isomerization on phospho-Ser/Thr-Pro bonds (8). Thr48 and Thr67 of cdc25C interact directly with the WW domain of Pin1 when these sites are phosphorylated (9). Thr48 phosphorylation also mediates binding to CKS/p13SUC1 (10).
- Jessus, C. and Ozon, R. (1995) Prog. Cell Cycle Res. 1, 215-228.
- Peng, C.Y. et al. (1997) Science 277, 1501-1505.
- Kumagai, A. and Dunphy, W.G. (1999) Genes Dev. 13, 1067-1072.
- Blasina, A. et al. (1999) Curr. Biol. 9, 1-10.
- Furnari, B. et al. (1999) Mol. Biol. Cell 10, 833-845.
- Izumi, T. and Maller, J.L. (1993) Mol. Biol. Cell 4, 1337-1350.
- Stukenberg, P. T. et al. (2001) Mol. Cell 7, 1071-1083.
- Yaffe, M. B. et al. (1997) Science 278, 1957-1960.
- Lu, P. J. et al. (1999) Science 283, 1325-1328.
- Landrieu, I. et al. (2001) J. Biol. Chem 276, 1434-1438.
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