Phospho-CXCR4 (Ser339) Antibody #59028
- WB
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 45-60 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.
Species predicted to react based on 100% sequence homology:
Source / Purification
Background
CXCR4 is phosphorylated at Ser339 by G protein-coupled receptor kinase 6 upon treatment of cells with CXCL12 (5). Phosphorylation of CXCR4 at Ser339 has also been observed following epidermal growth factor and phorbol ester treatment (6). TCR-mediated transactivation of CXCR4 at Ser339 activates a PREX1-Rac1-signaling pathway that stabilizes interleukin-2, -4, and -10 messenger RNA transcripts (7).
- Blanchet, X. et al. (2012) Front Immunol 3, 175.
- Döring, Y. et al. (2014) Front Physiol 5, 212.
- Debnath, B. et al. (2013) Theranostics 3, 47-75.
- Teicher, B.A. and Fricker, S.P. (2010) Clin Cancer Res 16, 2927-31.
- Busillo, J.M. et al. (2010) J Biol Chem 285, 7805-17.
- Woerner, B.M. et al. (2005) Cancer Res 65, 11392-9.
- Kremer, K.N. et al. (2017) Blood 130, 982-994.
Limited Uses
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