Render Target: STATIC
Render Timestamp: 2024-11-01T10:53:39.656Z
Commit: 23cb9f61fe67e1e9093fd644a533c4ff516a6463
XML generation date: 2024-08-01 15:30:13.109
Product last modified at: 2024-08-09T17:00:08.102Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

Phospho-dCK (Ser74) Antibody #40371

Filter:
  • WB

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 30
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Phospho-dCK (Ser74) Antibody recognizes endogenous levels of dCK protein only when phosphorylated at Ser74.

    Species Reactivity:

    Human

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser74 of human dCK protein. Antibodies are purified by peptide affinity chromatography.

    Background

    Deoxycytidine kinase (dCK) catalyzes the rate-limiting step in the nucleoside salvage pathway. dCK phosphorylates salvaged deoxycytidine (dC) to generate deoxycytidine monophosphate (dCMP), a precursor for both deoxycytidine triphosphate (dCTP) and deoxythymidine triphosphate (dTTP) pools (1). dCK also phosphorylates deoxyadenosine (dA) and deoxyguanosine (dG) to generate dA monophosphate and dG monophosphate, respectively. Gemcitabine is an anticancer nucleoside that is a dC analog. It is a pro-drug and must be phosphorylated by dCK to an active form. dCK is phosphorylated on Ser74 by the DNA damage signaling kinases ATM and ATR, and this phosphorylation increases dCK activity (2,3).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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