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Phospho-Drosophila Stat92E (Tyr711) Antibody #9357

Inquiry Info. # 9357

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    Product Specifications

    REACTIVITY
    SENSITIVITY
    MW (kDa) 84
    SOURCE Rabbit

    Product Information

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Specificity / Sensitivity

    Phospho-Drosophila Stat92E (Tyr711) Antibody detects endogenous levels of Drosophila Stat92E only when phosphorlated on tyrosine 711.

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Tyr711 of Stat92E. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    The Stat3 transcription factor is an important signaling molecule for many cytokines and growth factor receptors (1) and is required for murine fetal development (2). Research studies have shown that Stat3 is constitutively activated in a number of human tumors (3,4) and possesses oncogenic potential (5) and anti-apoptotic activities (3). Stat3 is activated by phosphorylation at Tyr705, which induces dimerization, nuclear translocation, and DNA binding (6,7). Transcriptional activation seems to be regulated by phosphorylation at Ser727 through the MAPK or mTOR pathways (8,9). Stat3 isoform expression appears to reflect biological function as the relative expression levels of Stat3α (86 kDa) and Stat3β (79 kDa) depend on cell type, ligand exposure, or cell maturation stage (10). It is notable that Stat3β lacks the serine phosphorylation site within the carboxy-terminal transcriptional activation domain (8).
    In Drosophila, Stat92E plays a critical role during development (9). Activated Stat92E, phosphorylated at Tyr711, can be observed at different developmental stages including the tracheal pits, elongating intestinal tracks, and growing axons (10). In Drosophila, the receptor tyrosine kinase Torso activates both Stat92E and Ras during primordial germ cell development, and that activation is required for germ cell proliferation and invasive migration (11).

    Alternate Names

    CG4257; MARE; marelle; mrL; Signal transducer and transcription activator; Stat; STAT5A; Stat92E

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