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Phospho-Estrogen Receptor α Antibody Sampler Kit

Phospho-Estrogen Receptor α Antibody Sampler Kit #9924

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Product Information

Kit Usage Information

Protocols

2511: Western Blotting, Immunohistochemistry (Paraffin)
2517: Western Blotting
5587: Western Blotting
7074: Western Blotting
7076: Western Blotting
8644: Western Blotting, Immunoprecipitation (Agarose), ChIP Magnetic, Chromatin IP-seq, CUT&RUN Assay

Product Description

The Phospho-Estrogen Receptor α Antibody Sampler Kit provides an economical means to evaluate the activation status of ERα, including phosphorylation of Ser104/106, Ser167, and Ser118. The monoclonal control ERα antibody is also included to detect total Estrogen Receptor α levels. The kit contains enough primary antibody to perform four Western blot experiments.

Background

Estrogen receptor α (ERα), a member of the steroid receptor superfamily, contains highly conserved DNA binding and ligand binding domains (1). Through its estrogen-independent and estrogen-dependent activation domains (AF-1 and AF-2, respectively), ERα regulates transcription by recruiting coactivator proteins and interacting with general transcriptional machinery (2). Phosphorylation at multiple sites provides an important mechanism to regulate ERα activity (3-5). Ser104, 106, 118, and 167 are located in the amino-terminal transcription activation function domain AF-1, and phosphorylation of these serine residues plays an important role in regulating ERα activity. Ser118 may be the substrate of the transcription regulatory kinase CDK7 (5). Ser167 may be phosphorylated by p90RSK and Akt (4,6). According to the research literature, phosphorylation at Ser167 may confer tamoxifen resistance in breast cancer patients (4).

Alternate Names

Database Links

UniProt ID: P03372

Entrez-Gene Id: 2099

Limited Uses

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For Research Use Only. Not for Use in Diagnostic Procedures.

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