Render Target: STATIC
Render Timestamp: 2024-12-02T10:40:44.457Z
Commit: cd2fae6ca3f811b1ddb1df24ac291ed56d5d501b
XML generation date: 2024-08-01 15:27:27.357
Product last modified at: 2024-11-28T12:00:11.069Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

Phospho-FRS2-α (Tyr196) Antibody #3864

Filter:
  • WB

    Supporting Data

    REACTIVITY H M
    SENSITIVITY Endogenous
    MW (kDa) 85
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Phospho-FRS2-α (Tyr196) Antibody detects endogenous levels of FRS2-α only when phosphorylated at tyrosine 196. The antibody may also detect a non-specific band at 65kDa.

    Species Reactivity:

    Human, Mouse

    The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.

    Species predicted to react based on 100% sequence homology:

    Rat

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr196 of human FRS2-α. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    Fibroblast growth factor receptor substrate 2 (FRS2, also called Suc-associated neurotrophic factor-induced tyrosine-phosphorylated target or SNT) participates in the transmission of extracellular signals from the fibroblast growth factor receptor (FGFR). FGFR activation leads to tyrosine phosphorylation of FRS2 (1). Two FRS2 family members have been identified, FRS2-α (SNT1) and FRS2-β (SNT2) (2), which are phosphorylated by these receptor tyrosine kinases (RTKs). Once phosphorylated, they recruit SH2 domain-containing proteins, including Grb2 and SHP-2 (3,4), mediating downstream signaling. Tyr436 is required for efficient SHP-2 recruitment (5), whereas Tyr196 functions as a docking site for Grb2-Sos complexes (6).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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