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Phospho-HER4/ErbB4 (Tyr1284)/EGFR (Tyr1173) (21A9) Rabbit mAb

Phospho-HER4/ErbB4 (Tyr1284)/EGFR (Tyr1173) (21A9) Rabbit mAb #4757

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Supporting Data

REACTIVITY	H
SENSITIVITY	Endogenous
MW (kDa)	180
Source/Isotype	Rabbit

Application Key:

WB-Western Blotting

Species Cross-Reactivity Key:

H-Human

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

Phospho-HER4/ErbB4 (Tyr1284)/EGFR (Tyr1173) (21A9) Rabbit mAb detects endogenous levels of HER4/ErbB4 protein only when phosphorylated on Tyr1284 and EGFR only when phosphorylated on Tyr1173. This antibody may cross-react with other tyrosine-phosphorylated proteins.

Species Reactivity:

Human

The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.

Species predicted to react based on 100% sequence homology:

Mouse, Rat

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr1284 of human HER4/ErbB4.

Background

Research studies have implicated the HER/ErbB receptor tyrosine kinase family in normal development, cardiac function and cancer (1,2). HER4/ErbB4, like other family members, has four ectodomains, a single transmembrane domain and a cytoplasmic tail containing the active tyrosine kinase domain (3). By binding to neuregulins and/or EGF family ligands, ErbB4 forms either a homodimer or heterodimer with other ErbB family members, which results in receptor activation and signaling (3). ErbB4 is ubiquitously expressed with the highest expression occurring in brain and heart. The expression of ErbB4 in breast cancer, pediatric brain cancer and other types of carcinomas has been reported in research studies suggesting that ErbB4 expression is involved in both normal tissue development and carcinogenesis (3).

Database Links

UniProt ID: Q15303

Entrez-Gene Id: 2066

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For Research Use Only. Not For Use In Diagnostic Procedures.

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