Name: Phospho-Insulin/IGF Receptor Antibody Sampler Kit
Brand: Cell Signaling Technology
Price: 476.00 USD
Availability: InStock

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Phospho-Insulin/IGF Receptor Antibody Sampler Kit: Image 1 — Western blot analysis of extracts from 3T3-L1 adipocytes, untreated or insulin-treated (100 nM for the indicated times), using Phospho-IGF-I Receptor β (Tyr1131)/Insulin Receptor β (Tyr1146) Antibody (upper) or control IR antibody (lower).

Phospho-Insulin/IGF Receptor Antibody Sampler Kit: Image 2 — Western blot analysis of untreated and IGF-treated Hela cell extracts as well as untreated and insulin-treated H-4-II-E cell extracts using Phospho-IGF-I-Receptor beta (Tyr1135/1136)/Insulin Receptor beta (Tyr1150/1151)(19H7) Rabbit mAb

Phospho-Insulin/IGF Receptor Antibody Sampler Kit: Image 3 — Western blot analysis of extracts from various cell lines using Insulin Receptor β (4B8) Rabbit mAb.

Phospho-Insulin/IGF Receptor Antibody Sampler Kit: Image 4 — Western blot analysis of extracts from MCF7 cells, untreated or stimulated with IGF-I, using Phospho-IGF-I Receptor β (Tyr1135) (DA7A8) Rabbit mAb (upper) and IGF-I Receptor β Antibody #3027 (lower).

Phospho-Insulin/IGF Receptor Antibody Sampler Kit: Image 5 — Western blot analysis of extracts from MCF-7 cells, untreated or IGF-I-treated, using Phospho-IGF-I Receptor β (Tyr980) (C14A11) Rabbit mAb (upper) and IGF-I Receptor β (111A9) Rabbit mAb #3018 (lower).

Phospho-Insulin/IGF Receptor Antibody Sampler Kit: Image 6 — After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO^® is added and emits light during enzyme catalyzed decomposition.

Phospho-Insulin/IGF Receptor Antibody Sampler Kit: Image 7 — Western blot analysis of extracts from 293 (IGF-I receptor β+) and SK-UT-1 (IGF-I receptor β-) cells using IGF-I Receptor β (D23H3) XP^® Rabbit mAb (upper) or β-Actin Antibody #4967 (lower).

Phospho-Insulin/IGF Receptor Antibody Sampler Kit: Image 8 — Western blot analysis of extracts from 293 cells, untreated or IGF-I-treated (100 nM for 2 minutes), using Phospho-IGF-I Receptor β (Tyr1131)/Insulin Receptor β (Tyr1146) Antibody (upper) or control IGF-I Receptor antibody (lower).

Phospho-Insulin/IGF Receptor Antibody Sampler Kit: Image 9 — Phospho-IGF-I Receptor
β (Tyr1135/1136)/Insulin Receptor β (Tyr1150/1151) (19H7) Rabbit mAb specifically binds to tyrosine phosphorylated IGF-1 and insulin receptors, but not other phosphorylated tyrosine kinases. Western blot analysis of of extracts from cells expressing different activated tyrosine kinase proteins, using Phospho-IGF-I Receptor β (Tyr1135/1136)/Insulin Receptor β(Tyr1150/1151) (19H7) Rabbit mAb (upper) or Phospho-Tyrosine Mouse mAb (P-Tyr-100) #9411 (lower).

Phospho-Insulin/IGF Receptor Antibody Sampler Kit: Image 10 — Immunprecipitation of Insulin Receptor beta from insulin treated mIMCD-3 cell extracts using Insulin Receptor beta antibody (Lane 1) Lane 2: No antibody control. Lane 3: Input control.

Phospho-Insulin/IGF Receptor Antibody Sampler Kit: Image 11 — Confocal immunofluorescent analysis of MCF7 (left) and SK-UT-1 (right) cells using IGF-I Receptor β (D23H3) XP^® Rabbit mAb (green). Blue pseudocolor = DRAQ5^® #4084 (fluorescent DNA dye).

Phospho-Insulin/IGF Receptor Antibody Sampler Kit: Image 12 — Flow cytometric analysis of fixed and permeabilized SK-UT-1 cells (blue, negative) and MCF7 cells (green, positive) using IGF-I Receptor β (D23H3) XP^® Rabbit mAb (solid lines) or a concentration-matched Rabbit (DA1E) mAb IgG XP^® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')₂ Fragment (Alexa Fluor^® 488 Conjugate) #4412 was used as a secondary antibody.

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To Purchase # 8338

Cat. #	Size	Qty.	Price
8338T	1 Kit (6 x 20 microliters)		$ 476

Product Includes
Related Products

Product Includes	Quantity	Applications	Reactivity	MW(kDa)	Isotype
Phospho-IGF-I Receptor β (Tyr1135) (DA7A8) Rabbit mAb 3918	20 µl	WB	H M R	95	Rabbit IgG
Phospho-IGF-I Receptor β (Tyr1131)/Insulin Receptor β (Tyr1146) Antibody 3021	20 µl	WB IP	H M R	95	Rabbit
Phospho-IGF-I Receptor β (Tyr1135/1136)/Insulin Receptor β (Tyr1150/1151) (19H7) Rabbit mAb 3024	20 µl	WB	H M R	95	Rabbit IgG
Phospho-IGF-I Receptor β (Tyr980) (C14A11) Rabbit mAb 4568	20 µl	WB	H M R	95	Rabbit IgG
Insulin Receptor β (4B8) Rabbit mAb 3025	20 µl	WB IP	H M R	95	Rabbit IgG
IGF-I Receptor β (D23H3) XP^® Rabbit mAb 9750	20 µl	WB IP IF F	H M R Mk	95	Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074	100 µl	WB			Goat

Kit Usage Information

Protocols

3021: Western Blotting, Immunoprecipitation (Magnetic)
3024: Western Blotting
3025: Western Blotting, Immunoprecipitation (Agarose)
3918: Western Blotting
4568: Western Blotting
7074: Western Blotting
9750: Western Blotting, Immunoprecipitation (Agarose), Immunofluorescence, Flow Triton Permeabilization (Rabbit)

Product Description

The Phospho-Insulin/IGF Receptor Antibody Sampler Kit provides an economical means of evaluating total Insulin Receptor and IGF-I Receptor β protein levels as well as Insulin and IGF-I Receptor β phosphorylated at specific sites. The kit includes enough antibody to perform two western blot experiments with each primary antibody.

Specificity / Sensitivity

Each antibody in the Phospho-Insulin/IGF Receptor Antibody Sampler Kit recognizes endogenous levels of the specific target protein, with activation state-specific antibodies recognizing target proteins only when phosphorylated at the indicated residues. IGF-I Receptor ß (D23H3) XP® Rabbit mAb detects endogenous levels of total IGF-I receptor ß protein. This antibody does not cross-react with insulin receptor.Phospho-IGF-I Receptor beta (Tyr1135) (DA7A8) Rabbit mAb detects endogenous levels of IGF-I receptor only when phosphorylated at Tyr1135. This antibody cross-reacts with Tyr1150 of insulin receptor and may also cross-react with other overexpressed related tyrosine-phosphorylated tyrosine kinases. Phospho-IGF-I Receptor ß (Tyr1131)/Insulin Receptor ß (Tyr1146) Antibody detects endogenous levels of Tyr1131-phosphorylated IGF-I receptor and Tyr1146-phosphorylated insulin receptor. This antibody cross-reacts with activated PDGF, FGF and EGF receptors; ErbB2; and c-Met. Phospho-IGF-I Receptor ß (Tyr1135/1136)/Insulin Receptor ß (Tyr1150/1151) (19H7) Rabbit mAb detects endogenous levels of IGF-I receptor and insulin receptor only when phosphorylated at tyrosine 1135/1136 or tyrosine 1150/1151, and does not cross-react with other related tyrosine-phosphorylated tyrosine kinases. Phospho-IGF-I Receptor ß (Tyr980) (C14A11) Rabbit mAb detects endogenous levels of IGF-I receptor ß protein only when phosphorylated at Tyr980, and may cross-react with activated insulin receptors and FLT3. Insulin Receptor ß (4B8) Rabbit mAb detects endogenous levels of total insulin receptor ß and does not cross-react with IGF-I receptor ß.

Source / Purification

Monoclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the carboxy-terminal residues of human IGF-IR β or residues surrounding Tyr960 of human insulin receptor β. Activation state-specific monoclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr1135 of human IGF-I Receptor β, Tyr1135/1136 of human IGF-I Receptor β, or Tyr980 of human IGF-I Receptor β. Activation state-specific polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues of human IGF-I Receptor β. Polyclonal antibodies are purified by protein A and peptide affinity chromatography.

Background

Type I insulin-like growth factor receptor (IGF-IR) is a transmembrane receptor tyrosine kinase that is widely expressed in many cell lines and cell types within fetal and postnatal tissues (1-3). Receptor autophosphorylation follows binding of the IGF-I and IGF-II ligands. Three tyrosine residues within the kinase domain (Tyr1131, Tyr1135, and Tyr1136) are the earliest major autophosphorylation sites (4). Phosphorylation of these three tyrosine residues is necessary for kinase activation (5,6). Insulin receptors (IRs) share significant structural and functional similarity with IGF-I receptors, including the presence of an equivalent tyrosine cluster (Tyr1146/1150/1151) within the kinase domain activation loop. Tyrosine autophosphorylation of IRs is one of the earliest cellular responses to insulin stimulation (7). Autophosphorylation begins with phosphorylation at Tyr1146 and either Tyr1150 or Tyr1151, while full kinase activation requires triple tyrosine phosphorylation (8).

Pathways & Proteins

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Database Links

UniProt ID: P08069

Entrez-Gene Id: 3480

View in PhosphoSitePlus^®

Limited Uses

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Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.

Phospho-Insulin/IGF Receptor Antibody Sampler Kit #8338