Phospho-IRF-7 (Ser471/472) Antibody #5184
- WB
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Transfected Only |
| MW (kDa) | 65 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Protocol
Specificity / Sensitivity
Phospho-IRF-7 (Ser471/472) Antibody detects transfected levels of IRF-7 when phosphorylated at Ser471 and 472.
Species Reactivity:
Human
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser471/472 of human IRF-7 protein. Antibodies were purified by protein A and peptide affinity chromatography.
Background
Interferon regulatory factors (IRFs) comprise a family of transcription factors that function within the Jak/Stat pathway to regulate interferon (IFN) and IFN-inducible gene expression in response to viral infection (1). IRFs play an important role in pathogen defense, autoimmunity, lymphocyte development, cell growth, and susceptibility to transformation. The IRF family includes nine members: IRF-1, IRF-2, IRF-9/ISGF3γ, IRF-3, IRF-4 (Pip/LSIRF/ICSAT), IRF-5, IRF-6, IRF-7, and IRF-8/ICSBP. All IRF proteins share homology in their amino-terminal DNA-binding domains. IRF family members regulate transcription through interactions with proteins that share similar DNA-binding motifs, such as IFN-stimulated response elements (ISRE), IFN consensus sequences (ICS), and IFN regulatory elements (IRF-E) (2).
IRF-7, which is functionally similar to IRF-3, is preferentially expressed in lymphoid cells and induced by virus, LPS, and IFN-α (3-5). IRF-7 plays an essential role in the induction of type I interferon in response viral infection (6-8). Like IRF-3, IRF-7 is regulated at multiple serine phosphorylation sites near its carboxyl terminus, which are required for nuclear translocation, DNA binding, and transcriptional activity (9-11).
- Taniguchi, T. et al. (2001) Annu Rev Immunol 19, 623-55.
- Honda, K. and Taniguchi, T. (2006) Nat Rev Immunol 6, 644-58.
- Au, W.C. et al. (1998) J Biol Chem 273, 29210-7.
- Wathelet, M.G. et al. (1998) Mol Cell 1, 507-18.
- Marié, I. et al. (1998) EMBO J 17, 6660-9.
- Sato, M. et al. (2000) Immunity 13, 539-48.
- Honda, K. et al. (2005) Nature 434, 772-7.
- Colina, R. et al. (2008) Nature 452, 323-8.
- Lin, R. et al. (2000) J Biol Chem 275, 34320-7.
- Yang, H. et al. (2003) J Biol Chem 278, 15495-504.
- Caillaud, A. et al. (2005) J Biol Chem 280, 17671-7.
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