|97999T||1 Kit (8 x 20 microliters)||
|Jak1 (6G4) Rabbit mAb 3344||20 µl||
||H M R||130||Rabbit IgG|
|Phospho-Jak1(Tyr1034/1035) (D7N4Z) Rabbit mAb 74129||20 µl||
||H M||130||Rabbit IgG|
|Jak2 (D2E12) XP® Rabbit mAb 3230||20 µl||
||H M R||125||Rabbit IgG|
|Phospho-Jak2 (Tyr1008) (D4A8) Rabbit mAb 8082||20 µl||
||H M||125||Rabbit IgG|
|Jak3 (D1H3) Rabbit mAb 8827||20 µl||
|Phospho-Jak3 (Tyr980/981) (D44E3) Rabbit mAb 5031||20 µl||
||H M||115||Rabbit IgG|
|Tyk2 (D4I5T) Rabbit mAb 14193||20 µl||
||H Mk||134||Rabbit IgG|
|Phospho-Tyk2 (Tyr1054/1055) (D7T8A) Rabbit mAb 68790||20 µl||
|Anti-rabbit IgG, HRP-linked Antibody 7074||100 µl||
Monoclonal antibodies are produced by immunizing rabbits with synthetic peptides corresponding to Ile800 of Jak1, Pro841 of Jak2, and the carboxy terminus of Jak3 and Tyk2. Phosphorylation-specific monoclonal antibodies are produced by immunizing rabbits with synthetic peptides corresponding to Tyr1034/1035 of Jak1, Tyr1008 of Jak2, Tyr980/981 of Jak3, and Tyr1054/1055 of Tyk2.
Members of the Janus family of tyrosine kinases (Jak1, Jak2, Jak3, and Tyk2) are activated by ligands binding to a number of associated cytokine receptors (1). Upon cytokine receptor activation, Jak proteins become autophosphorylated and phosphorylate their associated receptors to provide multiple binding sites for signaling proteins. These associated signaling proteins, such as Stats (2), Shc (3), insulin receptor substrates (4), and focal adhesion kinase (FAK) (5), typically contain SH2 or other phosphotyrosine-binding domains.
Activation of Jak kinases upon cytokine receptor binding is associated with tyrosine phosphorylation within their activation loops, including Tyr1034/1035 of Jak1, Tyr1007/1008 of Jak2, Tyr980/981 of Jak3, and Tyr1054/1055 of Tyk2. Many studies have indicated that various cytokine receptors have clear preferences that utilize distinct Jak family members. Aberrant regulation of Jak signaling is associated with a number of diseases, including myeloproliferative neoplasms, leukemia, and inflammatory disease (6).
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