Render Target: STATIC
Render Timestamp: 2024-10-15T09:39:10.279Z
Commit: 56767fe525c928647c8401233a175d0d607d385d
XML generation date: 2024-04-05 20:22:33.406
Product last modified at: 2024-10-04T12:00:13.065Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

Phospho-KIF1B (Ser1487) Antibody #11993

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 140, 200
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Phospho-KIF1B (Ser1487) Antibody recognizes endogenous levels of KIF1B protein only when phosphorylated at Ser1487.

    Species Reactivity:

    Human

    The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.

    Species predicted to react based on 100% sequence homology:

    Mouse, Rat, Monkey

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser1487 of human KIF1B protein. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    Kinesin-like protein KIF1B is a member of the kinesin 3 family of C-kinesins that are characterized by a kinesin-motor domain in the carboxy-terminal region. As part of the general mechanism of kinesin-mediated cellular transport, C-kinesins are known to drive microtubule plus and minus end motilities (1-3). KIF1B is implicated in the transport of synaptic proteins to the cell periphery in neuronal cell axons by interaction with Rab3 guanine nucleotide exchange factor (3). Mitochondria are also often transported in axons by KIF1B (3-4).
    Quantitative mass spectrometry profiling of mitotic phosphorylation revealed putative phosphorylation sites of KIF1B (5). Phospho-KIF1B (Ser1487) Antibody is directed at a site identified by Cell Signaling Technology® (CST) using PhosphoScan®, CST's LC-MS/MS platform for modification site discovery. Phosphorylation at Ser1487 was discovered using an Akt substrate antibody. Please visit PhosphoSitePlus®, CST's modification site knowledgebase, at www.phosphosite.org for more information.
    For Research Use Only. Not For Use In Diagnostic Procedures.
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