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Phospho-LIMK1 (Thr508)/LIMK2 (Thr505) (F5J2U) Rabbit Monoclonal Antibody #41004

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  • WB

    Product Specifications

    REACTIVITY H
    SENSITIVITY Transfected Only
    MW (kDa) 72
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Phospho-LIMK1 (Thr508)/LIMK2 (Thr505) (F5J2U) Rabbit Monoclonal Antibody recognizes transfected levels of LIMK1 protein only when phosphorylated at Thr508 and LIMK2 protein only when phosphorylated at Thr505. This antibody detects an approximately 100 kDa protein of unknown identity in some cell lines.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr508 of human LIMK1 protein.

    Background

    LIM kinases (LIMK1 and LIMK2) are serine/threonine kinases that have two zinc finger motifs, known as LIM motifs, in their amino-terminal regulatory domains (1). LIM kinases are involved in actin cytoskeletal regulation downstream of Rho-family GTPases, PAKs, and ROCK (2,3). PAK1 and ROCK phosphorylate LIMK1 or LIMK2 at the conserved Thr508 or Thr505 residues in the activation loop, increasing LIMK activity (3-5). Activated LIM kinases inhibit the actin depolymerization activity of cofilin by phosphorylation at the amino-terminal Ser3 residue of cofilin (6,7).

    Alternate Names

    LIM domain kinase 1; LIM domain kinase 2; LIM motif-containing protein kinase; LIMK; LIMK-1; LIMK-2; LIMK1; LIMK2

    For Research Use Only. Not for Use in Diagnostic Procedures.
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