|3316||Phospho-MAPKAPK-2 (Thr222) (9A7) Rabbit mAb||
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Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Phospho-MAPKAPK-2 (Thr222) Antibody detects endogenous levels of MAPKAPK-2 protein only when phosphorylated at threonine 222. It may cross-react with MAPKAPK-3 phosphorylated at threonine 200 and MAPKAPK-5 phosphorylated at threonine 182.
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr222 of human MAPKAPK- 2. Antibodies are purified by protein A and peptide affinity chromatography.
In response to cytokines, stress, and chemotactic factors, MAP kinase-activated protein kinase 2 (MAPKAPK-2) is rapidly phosphorylated and activated. It has been shown that MAPKAPK-2 is a direct target of p38 MAPK (1). Multiple residues of MAPKAPK-2 are phosphorylated in vivo in response to stress. However, only four residues (Thr25, Thr222, Ser272, and Thr334) are phosphorylated by p38 MAPK in an in vitro kinase assay (2). Phosphorylation at Thr222, Ser272, and Thr334 appears to be essential for the activity of MAPKAPK-2 (2). Thr25 is phosphorylated by p42 MAPK in vitro, but is not required for the activation of MAPKAPK-2 (2).
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