Render Target: STATIC
Render Timestamp: 2024-12-09T12:02:46.424Z
Commit: 224419269841c11382c4555dbee545259bf6c379
XML generation date: 2024-08-01 15:31:17.389
Product last modified at: 2024-08-02T20:45:06.990Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

Phospho-Myelin Basic Protein (Ser295) Antibody #45316

Filter:
  • WB

    Supporting Data

    REACTIVITY H M R
    SENSITIVITY Endogenous
    MW (kDa) 12-18
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Phospho-Myelin Basic Protein (Ser295) Antibody recognizes endogenous levels of myelin basic protein only when phosphorylated at Ser295, Ser295/Ser297, Ser295/Ser299, or Ser295/Ser297/Ser299. This antibody does not detect phosphorylation at Ser297 or Ser299 in the absence of phosphorylation at Ser295. This antibody also recognizes a non-specific band of unknown origin at 45 kDa.

    Species Reactivity:

    Human, Mouse, Rat

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser295 of human myelin basic protein. Antibodies are purified by peptide affinity chromatography.

    Background

    Myelin basic protein (MBP) is an abundant central nervous system (CNS) myelin membrane protein that plays an important role in nerve myelination. Myelin sheaths are multi-layered membranes derived from oligodendrocytes that increase the conduction velocity of axonal impulses. MBP helps to adhere the cytoplasmic leaflets of adjacent oligodendrocyte membranes to one another. Several splice variants of MBP are expressed in cells and the protein is modified following translation (i.e., phosphorylation, methylation), which suggests additional membrane adhesion functions (1).
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