Render Target: STATIC
Render Timestamp: 2024-11-06T11:01:56.804Z
Commit: 642d75590e907c0f7dfc7c6e3b846bcc0b02197c
XML generation date: 2024-08-01 15:28:28.997
Product last modified at: 2024-10-31T22:15:14.622Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

Phospho-Parkin (Ser65) Antibody #36866

Filter:
  • WB

    Supporting Data

    REACTIVITY H
    SENSITIVITY Transfected Only
    MW (kDa) 50
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Phospho-Parkin (Ser65) Antibody recognizes transfected levels of Parkin protein only when phosphorylated at Ser65.

    Species Reactivity:

    Human

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser65 of human Parkin protein. Antibodies are purified by peptide affinity chromatography.

    Background

    Parkin is a protein of 465 amino acids with an amino-terminal ubiquitin domain and a carboxy-terminal RING-box (1). In the case of autosomal recessive juvenile Parkinsonism (AR-JP), deletions have been found in the gene on chromosome 6 encoding the protein Parkin (2).
    Parkin is phosphorylated by PINK1 at site Ser65. Phosphorylation at this site has been shown to be necessary for Parkin activation in vivo (3).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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