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Phospho-PKD/PKC mu (Ser910) (E7M8C) Rabbit Monoclonal Antibody #75148

Filter:
  • WB

    Product Specifications

    REACTIVITY H M
    SENSITIVITY Endogenous
    MW (kDa) 115
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Phospho-PKD/PKC mu (Ser910) (E7M8C) Rabbit Monoclonal Antibody detects endogenous levels of PKD/PKCμ protein only when phosphorylated at Ser910.

    Species Reactivity:

    Human, Mouse

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser910 of human PKD/PKCμ protein. This site is homologous to Ser916 in mouse sequence.

    Background

    Activation of PKC is one of the earliest events in a cascade leading to a variety of cellular responses, such as secretion, gene expression, proliferation, and muscle contraction (1,2). Protein kinase D (PKD), also called PKCμ, is a serine/threonine kinase whose activation is dependent on the phosphorylation of two activation loop sites, Ser744 and Ser748, via a PKC-dependent signaling pathway (3-5). In addition to the two activation loop sites, the carboxy-terminal Ser916 has been identified as an autophosphorylation site for PKD/PKCμ. Phosphorylation at Ser916 correlates with PKD/PKCμ catalytic activity (6).

    Alternate Names

    CHDED; KPCD1; nPKC-D1; nPKC-mu; PKC-MU; PKCM; PKD; PKD1; PRKCM; PRKD1; Protein kinase C mu type; protein kinase C, mu; Protein kinase D; protein kinase D1; Serine/threonine-protein kinase D1

    For Research Use Only. Not for Use in Diagnostic Procedures.
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