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Primary Antibodies /
Phospho-PKR (Thr446/451) Antibody

Phospho-PKR (Thr446/451) Antibody #3071

Inquiry Info. # 3071

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Product Specifications

REACTIVITY
SENSITIVITY
MW (kDa)	74
SOURCE	Rabbit

Product Information

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

Phospho-PKR (Thr446/451) Antibody detects endogenous levels of PKR only when phosphorylated at threonine 446 and 451.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr446/451 of human PKR. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Protein kinase R (PKR) is transcriptionally induced by interferon and activated by double-stranded RNA (dsRNA). PKR inhibits translation initiation through phosphorylation of the α subunit of the initiation factor eIF2 (eIF2α) and also controls the activation of several transcription factors, such as NF-κB, p53, and the Stats. In addition, PKR mediates apoptosis induced by many different stimuli, such as LPS, TNF-α, viral infection, and serum starvation (1,2). Activation of PKR by dsRNA results in PKR dimerization and autophosphorylation of Thr446 and Thr451 in the activation loop. Substitution of threonine for alanine at position 451 completely inactivated PKR, while a mutant with a threonine to alanine substitution at position 446 was partially active (3). Research studies have implicated PKR activation in the pathologies of neurodegenerative diseases, including Alzheimer's disease (4,5).

Alternate Names

double stranded RNA activated protein kinase; E2AK2; eIF-2A protein kinase 2; EIF2AK1; EIF2AK2; eukaryotic translation initiation factor 2 alpha kinase 2; Eukaryotic translation initiation factor 2-alpha kinase 2; Interferon-induced, double-stranded RNA-activated protein kinase; interferon-inducible elF2alpha kinase; Interferon-inducible RNA-dependent protein kinase; LEUDEN; MGC126524; P1/eIF-2A protein kinase; p68 kinase; PKR; PPP1R83; PRKR; Protein kinase R; Protein kinase RNA-activated; protein kinase, interferon-inducible double stranded RNA dependent; protein phosphatase 1, regulatory subunit 83; Tyrosine-protein kinase EIF2AK2

Database Links

UniProt ID: P19525

Entrez-Gene Id: 5610

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For Research Use Only. Not for Use in Diagnostic Procedures.

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