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Phospho-PPP1R14A/CPI-17 (Thr38) (F1V4V) Rabbit Monoclonal Antibody

Phospho-PPP1R14A/CPI-17 (Thr38) (F1V4V) Rabbit Monoclonal Antibody #98511

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Product Specifications

REACTIVITY	H R
SENSITIVITY	Endogenous
MW (kDa)	19, 17
Source/Isotype	Rabbit IgG

Application Key:

WB-Western Blotting

Species Cross-Reactivity Key:

H-Human
R-Rat

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

Phospho-PPP1R14A/CPI-17 (Thr38) (F1V4V) Rabbit Monoclonal Antibody recognizes endogenous levels of PPP1R14A/CPI-17 protein only when phosphorylated at Thr38. This antibody does not cross-react with PPP1R14B protein. This antibody detects a 100 kDa protein of unknown identity in some cell lines.

Species Reactivity:

Human, Rat

The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.

Species predicted to react based on 100% sequence homology:

Mouse

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr38 of human PPP1R14A/CPI-17 protein.

Background

Protein kinase C-potentiated inhibitor protein of 17 kDa (CPI-17), encoded by the protein phosphatase 1 (PP1) regulatory subunit 14A (PPP1R14A) gene, promotes smooth muscle contraction by inhibiting the activity of the PP1 holoenzyme, smooth muscle myosin phosphatase (MYPT1) (1). CPI-17 belongs to a family of proteins, including PPP1R14B/PHI-1, PPP1R14C/KEPI, and PPP1R14D/GEPI, that each specifically inhibits a subset of PP1 holoenzymes (1). CPI-17 expression, localization, and enzymatic activity are driven by a combination of epigenetic and posttranslational modifications, all of which have downstream effects on PP1-dependent cellular processes. CPI-17 is a substrate for PKC, ROCK1, ZIPK, MST3/MST4, and CaMKII, with established phosphorylation target sites at Ser12, Thr38, and Ser130 (1-6). Additional putative phosphorylation sites with as yet undefined upstream kinases may be regulated by luteinizing hormone signaling in rodent ovary (7). CPI-17 is translocated from the cytoplasm to the nucleus via the importin complex, and phosphorylation of CPI-17 at Ser12 disrupts the N-terminal nuclear localization signal (8). Nuclear CPI-17 inhibits PP1-mediated dephosphorylation of histone H3, and CPI-17 protein knockdown by siRNA decreased PANC-1 pancreatic carcinoma cell proliferation (8). Thr38 phosphorylation increases CPI-17 inhibitory activity 1,000-fold, and can be induced in vitro by upstream kinase agonists (9,10). In a pan-cancer analysis, PPP1R14A mRNA and protein expression were found to be decreased across a wide variety of cancer types, including breast, colon, and ovarian cancers, kidney renal clear cell carcinoma, lung adenocarcinoma, and uterine corpus endometrial carcinoma (11). The pan-cancer expression profile correlated with increased PPP1R14A promoter methylation (11). Increased PPP1R14A gene silencing by DNA methylation was also observed in nearly 80% of non-Hodgkin lymphoma patient samples relative to control B lymphocytes (12).

Alternate Names

17 kDa PKC-potentiated inhibitory protein of PP1; 17-KDa protein; 17-kDa PKC-potentiated inhibitory protein of PP1; 17-KDa protein; CPI-17; CPI17; PKC-potentiated inhibitory protein of PP1; PP14A; PPP1INL; PPP1R14A; Protein kinase C-potentiated inhibitor protein of 17 kDa; protein phosphatase 1 regulatory inhibitor subunit 14A; Protein phosphatase 1 regulatory subunit 14A; protein phosphatase 1, regulatory (inhibitor) subunit 14A

Database Links

UniProt ID: Q96A00

Entrez-Gene Id: 94274

Limited Uses

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For Research Use Only. Not for Use in Diagnostic Procedures.

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