Render Target: STATIC
Render Timestamp: 2024-12-13T11:56:29.351Z
Commit: 611277b6de3cd1bb065350b6ef8d63df412b7185
XML generation date: 2024-09-20 06:15:54.331
Product last modified at: 2024-11-06T13:30:18.204Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

Phospho-PRK1 (Thr774)/PRK2 (Thr816) Antibody #2611

Filter:
  • WB

    Supporting Data

    REACTIVITY H M R
    SENSITIVITY Endogenous
    MW (kDa) 120 Phospho-PRK1. 140 Phospho-PRK2.
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Phospho-PRK1 (Thr774)/ PRK2 (Thr816) Antibody detects endogenous levels of PRK1 and PRK2 only when phosphorylated at Thr774 or Thr816, respectively. This antibody also detects PKC zeta and lambda when phosphorylated at Thr 410 and 403, respectively.

    Species Reactivity:

    Human, Mouse, Rat

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr774 of human PRK1. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    The protein kinase C-related kinases (PRKs) are a subfamily of Ser/Thr-specific kinases with a catalytic domain highly homologous to the PKC family (1-3). They are effectors of Rho family GTPases (4-6) and are activated by fatty acids and phospholipids in vitro (7,8). Activation in vitro and in vivo involves the activation loop phosphorylation of PRK1 (Thr774)/PRK2 (Thr816) by PDK1 (9,10).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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