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Render Timestamp:
6/2/2026, 6:44:02 AM EDT
6/2/2026, 10:44:02 AM UTC
Commit: 7ed46ecc04b401f23a28df741b5078df405d23e4
XML generation date: 2026-05-06 03:26:41.778
Product last modified at: 2026-05-06T08:00:12.889Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
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Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Phospho-Rab12 (Ser106) (F4C6L) Rabbit Monoclonal Antibody #45036

Filter:
  • WB
  • IP

    Product Specifications

    REACTIVITY H M
    SENSITIVITY Endogenous
    MW (kDa) 27
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Phospho-Rab12 (Ser106) (F4C6L) Rabbit Monoclonal Antibody recognizes endogenous levels of Rab12 protein only when phosphorylated at Ser106.

    Species Reactivity:

    Human, Mouse

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser106 of human Rab12 protein.

    Background

    Rab12, a member of the Ras superfamily of small Rab GTPases, localizes to the trans-Golgi network (TGN), endosomes, and lysosomes to regulate various cellular protein trafficking. Reported protein trafficking functions of Rab12 include transporting the epidermal growth factor receptor (EGFR) from the TGN to the plasma membrane and transporting the transferrin receptor (TfR) from recycling endosomes to lysosomes (1-3). Rab12 is both a regulator and a substrate of Leucine-rich repeat kinase 2 (LRRK2). It recruits LRRK2 to the lysosomal membrane in response to damage, which enhances kinase activity and promotes Rab10 phosphorylation (4). In addition to its role in lysosomal damage response, the Rab12-LRRK2 complex regulates ciliary and centrosome homeostasis in the brain. As a substrate, Rab12 is phosphorylated by LRRK2 within its switch-II domain at Ser106. LRRK2 mutations associated with Parkinson's disease (PD), as well as the VPS35 D620N mutation linked to PD that lies upstream of LRRK2, drive kinase hyperactivity, increasing Rab12 phosphorylation (5-8). This phosphorylation site is a suggested blood-based biomarker for patients with a G2019S LRRK2 mutation (9). Elevated Rab12 phosphorylation at Ser106 is also observed in the brain of patients diagnosed with PD, dementia with Lewy bodies (DLB), Alzheimer’s disease (AD), Pick’s disease, progressive supranuclear palsy (PSP), and corticobasal degeneration (CBD) (10).

    Alternate Names

    FLJ45927; MGC104724; Putative Ras-related protein Rab-12; RAB12; RAB12, member RAS oncogene family; Ras-related protein Rab-12

    For Research Use Only. Not for Use in Diagnostic Procedures.
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