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Phospho-Rac1/Cdc42 (Ser71) (F4Q8K) Rabbit Monoclonal Antibody #71474

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  • WB
  • IP

    Product Specifications

    REACTIVITY H M R
    SENSITIVITY Endogenous
    MW (kDa) 28
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:100

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Phospho-Rac1/Cdc42 (Ser71) (F4Q8K) Rabbit Monoclonal Antibody recognizes endogenous levels of Rac1/Cdc42 protein only when phosphorylated at Ser71. This antibody also recognizes RhoA when phosphorylated at Ser73. This antibody detects a 100 kDa protein of unknown identity in some cell lines.

    Species Reactivity:

    Human, Mouse, Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser71 of human Rac1/Cdc42 protein.

    Background

    Rac and Cdc42 are members of the Rho-GTPase family. In mammals, Rac exists as three isoforms, Rac1, Rac2 and Rac3, which are highly similar in sequence. Rac1 and Cdc42, the most widely studied of this group, are ubiquitously expressed. Rac2 is expressed in cells of hematopoietic origin, and Rac3, while highly expressed in brain, is also found in many other tissues. Rac and Cdc42 play key signaling roles in cytoskeletal reorganization, membrane trafficking, transcriptional regulation, cell growth and development (1). GTP binding stimulates the activity of Rac/Cdc42, and the hydrolysis of GTP to GDP through the protein's intrinsic GTPase activity, rendering it inactive. GTP hydrolysis is aided by GTPase activating proteins (GAPs), while exchange of GDP for GTP is facilitated by guanine nucleotide exchange factors (GEFs). Another level of regulation is achieved through the binding of RhoGDI, a guanine nucleotide dissociation inhibitor, which retains Rho family GTPases, including Rac and Cdc42, in their inactive GDP-bound state (2,3).
    A putative Akt phosphorylation site at Ser71 of Rac1/cdc42 has been identified and confirmed by in vitro kinase assay (4). Phosphorylation at this site may inhibit GTP binding of Rac1, attenuating the signal transduction pathway downstream of Rac1 (4).

    Alternate Names

    CDC42; CDC42Hs; Cell division control protein 42 homolog; cell division cycle 42; cell division cycle 42 (GTP binding protein, 25kDa); Cell migration-inducing gene 5 protein; dJ224A6.1.1 (cell division cycle 42 (GTP-binding protein, 25kD)); dJ224A6.1.2 (cell division cycle 42 (GTP-binding protein, 25kD)); G25K; G25K GTP-binding protein; growth-regulating protein; GTP binding protein, 25kDa; GTP-binding protein, 25kD; MGC111543; MIG5; migration-inducing gene 5; MRD48; p21-Rac1; Rac family small GTPase 1; Rac-1; RAC1; Ras-like protein TC25; Ras-related C3 botulinum toxin substrate 1; ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1); rho family, small GTP binding protein Rac1; small GTP binding protein CDC42; TC-25; TC25; TKS

    For Research Use Only. Not for Use in Diagnostic Procedures.
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