Render Target: STATIC
Render Timestamp: 2024-10-15T09:56:08.504Z
Commit: 56767fe525c928647c8401233a175d0d607d385d
XML generation date: 2024-08-01 15:25:35.155
Product last modified at: 2024-06-11T20:15:08.613Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

Phospho-Shc (Tyr317) Antibody #2431

Filter:
  • WB

    Supporting Data

    REACTIVITY H M
    SENSITIVITY Endogenous
    MW (kDa) 50, 55, 70
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Phospho-Shc (Tyr317) Antibody detects Shc only when phosphorylated at Tyr317.

    Species Reactivity:

    Human, Mouse

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr317 of human Shc. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    SHC-transforming protein 1 (SHC1) is an adaptor protein to targets of activated tyrosine kinases. SHC1 is ubiquitously expressed and the gene is expressed in 2 transcripts, resulting in 3 isoforms with distinct functions and subcellular locations (1). The phosphorylated p46 and p52 isoforms couple activated receptor tyrosine kinases to Ras via the recruitment of the GRB2/SOS complex. The third isoform, p66, acts as a downstream target of the tumor suppressor p53 and is thereby implicated in oxidative stress response, tumor progression, and cancer cell proliferation (2,3). SHC1 has been identified to have prognostic and diagnostic value in multiple cancers, making it a potential biomarker (4).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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