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  3. Phospho-Smad Antibody Sampler Kit

Phospho-Smad Antibody Sampler Kit #9963

Inquiry Info. # 9963

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    Product Information

    Kit Usage Information

    Protocols

    Product Description

    The Phospho-Smad Antibody Sampler Kit contains reagents to investigate the activation of the TGF-β and BMP signaling pathways. The kit contains enough primary and secondary antibodies to perform four Western blot experiments per primary antibody.

    Background

    Members of the SMAD family of signal transduction molecules are components of a critical intracellular pathway that transmit TGF-β signals from the cell surface into the nucleus. Three distinct classes of SMADs have been defined: the receptor-regulated SMADs (R-SMADs), which include SMAD1, 2, 3, 5, and 9; the common-mediator SMAD (co-SMAD), SMAD4; and the antagonistic or inhibitory SMADs (I-SMADs), SMAD6 and 7 (1-5). Activated type I receptors associate with specific R-SMADs and phosphorylate them on a conserved carboxy-terminal SSXS motif. The phosphorylated R-SMADs dissociate from the receptor and form a heteromeric complex with SMAD4, initiating translocation of the heteromeric SMAD complex to the nucleus. Once in the nucleus, SMADs recruit a variety of DNA binding proteins that function to regulate transcriptional activity (6-8).

    Alternate Names

    BMP; MAD; psmad2; Smad; Smad1; smad158; Smad2; smad23; Smad3; Smad4; Smad5; smad6

    Limited Uses

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    For Research Use Only. Not for Use in Diagnostic Procedures.
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