Phospho-STING (Ser366) (D8K6H) Rabbit mAb #40818
- IF
- F
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| Source/Isotype | Rabbit IgG |
Application Key:
- IF-Immunofluorescence
- F-Flow Cytometry
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Immunofluorescence (Immunocytochemistry) | 1:800 - 1:1600 |
| Flow Cytometry (Fixed/Permeabilized) | 1:1600 |
Storage
For a carrier free (BSA and azide free) version of this product see product #99309.
Protocol
Specificity / Sensitivity
Phospho-STING (Ser366) (D8K6H) Rabbit mAb recognizes endogenous levels of STING protein only when phosphorylated at Ser366.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser366 of human STING protein.
Background
Stimulator of interferon genes (STING, TMEM173, MITA) is a transmembrane adaptor protein that is a critical component of the cellular innate immune response to pathogenic cytoplasmic DNA (1,2). STING is a ubiquitously expressed protein found predominantly in the ER (1). The enzyme cGAMP synthase (cGAS) produces the second messenger cyclic-GMP-AMP (cGAMP) in response to cytoplasmic DNA (3,4). cGAMP binds and activates STING (3,4). In addition, detection of cytoplasmic DNA by nucleic acid sensors, including DDX41 or IFI16, results in STING activation (5,6). Following activation, STING translocates with TBK1 to perinuclear endosomes (7). The TBK1 kinase phosphorylates and activates interferon regulatory factors (IRFs) and NF-κB, which leads to the induction of type I interferon and other immune response genes (1,2,7).
Following binding of cyclic dinucleotides, STING is phosphorylated by TBK1 at Ser366 (Ser365 in mouse), leading to IRF-3 activation and type I interferon upregulation (8).
- Ishikawa, H. and Barber, G.N. (2008) Nature 455, 674-8.
- Zhong, B. et al. (2008) Immunity 29, 538-50.
- Sun, L. et al. (2013) Science 339, 786-91.
- Wu, J. et al. (2013) Science 339, 826-30.
- Zhang, Z. et al. (2011) Nat Immunol 12, 959-65.
- Unterholzner, L. et al. (2010) Nat Immunol 11, 997-1004.
- Ishikawa, H. et al. (2009) Nature 461, 788-92.
- Liu, S. et al. (2015) Science 347, aaa2630.
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