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Phospho-Syntenin-1/MDA9 (Ser60) (F4T2K) Rabbit Monoclonal Antibody #51623

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  • WB
  • IP

    Product Specifications

    REACTIVITY H
    SENSITIVITY Transfected Only
    MW (kDa) 30
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:200

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Phospho-Syntenin-1/MDA9 (Ser60) (F4T2K) Rabbit Monoclonal Antibody recognizes transfected levels of syntenin-1/MDA9 protein only when phosphorylated at Ser60.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser60 of human syntenin-1/MDA9 protein.

    Background

    Syntenin-1, also known as MDA9 or SDCBP, is a member of the PDZ family of proteins (1), defined by the presence of a PDZ protein interaction domain. Syntenin-1, via its PDZ domain, functions as a scaffold adaptor protein that regulates numerous signal transduction pathways, including FAK, Src, TGF-βR1, EGFR, and IGF1R (2-5). Aberrantly elevated expression of syntenin-1 has been found in various cancers and has been linked to the growth and invasion of tumor cells (5-7). In the central nervous system, syntenin-1 has been shown to mediate neuron dendrite protrusion and spine formation through its interaction with syndecan and Rheb (8). Syntenin-1 is also a key component of the syndecan/syntenin/ALIX pathway, and it has been shown to contribute directly to the formation and internalization of exosomes (9-11).

    Syntenin-1/MDA9 has been shown to be phosphorylated at Ser61 by ULK1 in mouse, which is highly conserved with Ser60 in human (12).

    Alternate Names

    MDA-9; MDA9; melanoma differentiation associated protein-9; Melanoma differentiation-associated protein 9; Pro-TGF-alpha cytoplasmic domain-interacting protein 18; Scaffold protein Pbp1; SDCB1; SDCBP; ST1; SYCL; syndecan binding protein; syndecan binding protein (syntenin); Syndecan-binding protein 1; syntenin; Syntenin-1; TACIP18

    For Research Use Only. Not for Use in Diagnostic Procedures.
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