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96628
Phospho-Tau Family Antibody Sampler Kit

Phospho-Tau Family Antibody Sampler Kit #96628

Western Blotting Image 1

Western blot analysis of extracts from various cell lines and tissues using Tau (D1M9X) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

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Western Blotting Image 2

Western blot analysis of extracts from human cortex (lane 1), neonatal mouse brain, untreated (lane 2) or phosphatase-treated (lane 3), and fetal rat brain (lane 4), using Phospho-Tau (Ser404) (D2Z4G) Rabbit mAb (IF preferred) (upper), Tau (Tau46) Mouse mAb #4019 (middle) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

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Western Blotting Image 3

Western blot analysis of extracts from mouse and rat brain using Phospho-Tau (Ser416) (D7U2P) Rabbit mAb. The phospho-specificity of Phospho-Tau (Ser416) (D7U2P) Rabbit mAb was verified by peptide blocking using a phosphopeptide or non-phosphopeptide targeting residue Ser416.

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Western Blotting Image 4

Western blot analysis of extracts from neonatal and adult mouse brain using Phospho-Tau (Ser202) (D4H7E) Rabbit mAb. The phospho-specificity of the antibody was verified by blocking with a phospho- or nonphosphopeptide.

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Western Blotting Image 5

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

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Western Blotting Image 6

Western blot analysis of extracts from mouse brain and CAD cells, using Phospho-Tau (Ser396) (PHF13) Mouse mAb. The phospho-specificity of the antibody was verified by peptide blocking using no peptide, phospho-peptide or nonphospho-peptide.

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Western Blotting Image 7

Western blot analysis of extracts from human cortex (lane 1), untreated neonatal mouse brain (lane 2), phosphatase-treated neonatal mouse brain (lane 3), and fetal rat brain (lane 4), using Phospho-Tau (Ser404) (D2Z4G) Rabbit mAb (IHC preferred) (upper), Tau (Tau46) Mouse mAb #4019 (middle), and β-Actin (D6A8) Rabbit mAb #8457 (lower).

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IHC-P (paraffin) Image 8

Immunohistochemical analysis of paraffin-embedded human Alzheimer's brain using Tau (D1M9X) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).

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Western Blotting Image 9

Western blot analysis of extracts from MEF cells (lane 1) and mouse brain (lane 2) using Phospho-Tau (Ser404) (D2Z4G) Rabbit mAb (IF preferred) (upper), Tau (Tau46) Mouse mAb #4019 (middle) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

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Western Blotting Image 10

Western blot analysis of mouse brain lysate, untreated (-) or λ phosphatase-treated (+), using Phospho-Tau (Ser416) (D7U2P) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

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Western Blotting Image 11

Western blot analysis of extracts from mouse brain, untreated (-) or λ-phosphatase-treated (+), using Phospho-Tau (Ser202) (D4H7E) Rabbit mAb (upper) and β-Tubulin (D2N5G) Rabbit mAb #15115 (lower).

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IHC-P (paraffin) Image 12

Immunohistochemical analysis of paraffin-embedded human Alzheimer's brain using Phospho-Tau (Ser404) (D2Z4G) Rabbit mAb (IHC Preferred).

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IHC-P (paraffin) Image 13

Immunohistochemical analysis of paraffin-embedded human normal appendix using Tau (D1M9X) XP® Rabbit mAb.

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IF-IC Image 14

Confocal immunofluorescent analysis of mouse primary neurons using Phospho-Tau (Ser404) (D2Z4G) Rabbit mAb (IF preferred) (green). Blue pseudocolor = Hoescht 33342 #4082 (fluorescent DNA dye).

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IHC-P (paraffin) Image 15

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Phospho-Tau (Ser416) (D7U2P) Rabbit mAb.

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IHC-P (paraffin) Image 16

Immunohistochemical analysis of paraffin-embedded mouse colon, untreated (left) or λ phosphatase-treated (right), using Phospho-Tau (Ser404) (D2Z4G) Rabbit mAb (IHC Preferred).

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IHC-P (paraffin) Image 17

Immunohistochemical analysis of paraffin-embedded T-47D cell pellet (left, positive) or MDA-MB-231 cell pellet (right, negative) using Tau (D1M9X) XP® Rabbit mAb.

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IF-F Image 18

Confocal immunofluorescent analysis of Tg2576 mouse brain, untreated (left) or Lambda Protein Phosphatase-treated (right), using Phospho-Tau (Ser404) (D2Z4G) Rabbit mAb (IF preferred) (red) and GFAP (GA5) Mouse mAb #3670 (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

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IHC-P (paraffin) Image 19

Immunohistochemical analysis of paraffin-embedded mouse brain, untreated (left) or λ phosphatase-treated (right), using Phospho-Tau (Ser416) (D7U2P) Rabbit mAb.

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IHC-P (paraffin) Image 20

Immunohistochemical analysis of paraffin-embedded T-47D cell pellets (left, positive) and MDA-MB-231 cell pellets (right, negative) using Phospho-Tau (Ser404) (D2Z4G) Rabbit mAb (IHC Preferred).

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IHC-P (paraffin) Image 21

Immunohistochemical analysis of paraffin-embedded mouse lung using Tau (D1M9X) XP® Rabbit mAb.

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IHC-P (paraffin) Image 22

Immunohistochemical analysis of paraffin-embedded mouse brain using Phospho-Tau (Ser404) (D2Z4G) Rabbit mAb (IHC Preferred) in the presence of non-phosphorylated tau peptide (left) and antigen-specific phospho-tau (Ser404) peptide (right).

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IF-IC Image 23

Confocal immunofluorescent analysis of T-47D (positive, left) or MDA-MB-231 (negative, right) cells using Tau (D1M9X) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

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IF-F Image 24

Confocal immunofluorescent analysis of mouse striatum (left), hippocampus (middle), or midbrain (right) using Tau (D1M9X) XP® Rabbit mAb (green) and GFAP (GA5) Mouse mAb #3670 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

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Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Tau (D1M9X) XP® Rabbit mAb 46687 20 µl
  • WB
  • IHC
  • IF
H M R 50 to 80 Rabbit IgG
Phospho-Tau (Ser404) (D2Z4G) Rabbit mAb (IF preferred) 20194 20 µl
  • WB
  • IP
  • IF
H M R 50-80 Rabbit IgG
Phospho-Tau (Ser416) (D7U2P) Rabbit mAb 15013 20 µl
  • WB
  • IP
  • IHC
H M R 50-80 Rabbit IgG
Phospho-Tau (Ser202) (D4H7E) Rabbit mAb 39357 20 µl
  • WB
H M R 50-80 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
  • WB
Goat 
Anti-mouse IgG, HRP-linked Antibody 7076 100 µl
  • WB
Horse 
Phospho-Tau (Ser396) (PHF13) Mouse mAb 9632 20 µl
  • WB
M R 50-80 Mouse IgG2b
Phospho-Tau (Ser404) (D2Z4G) Rabbit mAb (IHC preferred) 35834 20 µl
  • WB
  • IHC
H M R 50-80 Rabbit IgG

The Phospho-Tau Family Antibody Sampler Kit provides an economical means of detecting the activation of Tau family members using phospho-specific and control antibodies. The kit includes enough antibody to perform two western blot experiments with each primary antibody.

Each antibody in the Phospho-Tau Family Antibody Sampler Kit detects endogenous levels of this target protein. Tau (D1M9X) XP® Rabbit mAb recognizes endogenous levels of total Tau protein. Phospho-Tau(Ser404) (D2Z4G) Rabbit mAb (IF preferred) recognizes endogenous levels of Tau protein when phosphorylated at Ser404. This antibody detects single phosphorylation at Ser404, dual phosphorylation at Ser400/Thr403/Ser404. This antibody does not detect peptides with single phosphorylation at Ser400 or Thr403. Phospho-Tau (Thr181) (D9F4G) Rabbit mAb recognizes endogenous levels of Tau protein only when phosphorylated at Thr181. Phospho-Tau (Ser202) (D4H7E) Rabbit mAb recognizes endogenous levels of Tau protein only when phosphorylated at Ser202. Phospho-Tau (Ser396) (PHF13) Mouse mAb recognizes endogenous levels of Tau protein only when phosphorylated at Ser396. Phospho-Tau (Ser416) (D7U2P) Rabbit mAb recognizes endogenous levels of Tau protein only when phosphorylated at Ser416.

Monoclonal antibodies are produced by immunizing rabbits with synthetic peptides corresponding to residues surrounding Asp430 of human Tau. Phosphorylation-specific monoclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to Ser400/Thr403/Ser404, Thr181, Ser202, Ser416 Tau, and Ser396 with human purified Tau (Hoffmann et al., 1997 -PMID 9201960).

Tau is a heterogeneous microtubule-associated protein that promotes and stabilizes microtubule assembly, especially in axons. Six isoforms with different amino-terminal inserts and different numbers of tandem repeats near the carboxy terminus have been identified, and tau is hyperphosphorylated at approximately 25 sites by Erk, GSK-3, and CDK5 (1,2). Phosphorylation decreases the ability of tau to bind to microtubules. Neurofibrillary tangles are a major hallmark of Alzheimer's disease; these tangles are bundles of paired helical filaments composed of hyperphosphorylated tau. In particular, phosphorylation at Ser396 by GSK-3 or CDK5 destabilizes microtubules. Furthermore, research studies have shown that inclusions of tau are found in a number of other neurodegenerative diseases, collectively known as tauopathies (1,3).

Investigators have shown that Tau phosphorylation at Ser404 destabilizes microtubules and that Tau is hyperphosphorylated at Ser404 in Alzheimer's disease (4-7). Research studies indicate that calcium-/calmodulin-dependent protease kinase II (CAM-kkinase II) is responsible for the phosphorylation of Tau at Ser416. Phosphorylated Tau protein is localized with neuronal soma or hippocampal neurons and immortalized GnRH neurons (8). Investigators have shown that Tau is phosphorylated during development and hyperphosphorylated at Ser202 in Alzheimer's disease (9).

  1. Johnson, G.V. and Stoothoff, W.H. (2004) J Cell Sci 117, 5721-9.
  2. Hanger, D.P. et al. (1998) J Neurochem 71, 2465-76.
  3. Bramblett, G.T. et al. (1993) Neuron 10, 1089-99.
  4. Hanger, D.P. et al. (1998) J Neurochem 71, 2465-76.
  5. Shiurba, R.A. et al. (1996) Brain Res 737, 119-32.
  6. Yamamoto, H. et al. (2005) J Neurochem 94, 1438-47.
  7. Goedert, M. et al. (1993) Proc Natl Acad Sci U S A 90, 5066-70.
  8. Evans, D.B. et al. (2000) J Biol Chem 275, 24977-83.
  9. Bertrand, J. et al. (2010) Neuroscience 168, 323-34.
Entrez-Gene Id
4137
Swiss-Prot Acc.
P10636-8
For Research Use Only. Not For Use In Diagnostic Procedures.

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XP is a registered trademark of Cell Signaling Technology, Inc.

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