Render Target: STATIC
Render Timestamp: 2024-11-05T09:40:02.134Z
Commit: 57f6e368eba1a427377652f2ad915d45d7f340a4
XML generation date: 2024-08-01 15:31:56.231
Product last modified at: 2024-10-30T12:45:29.358Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

Phospho-TFE3 (Ser321) Antibody #23816

Filter:
  • WB

    Supporting Data

    REACTIVITY H M R
    SENSITIVITY Endogenous
    MW (kDa) 76-90
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Phospho-TFE3 (Ser321) Antibody recognizes endogenous levels of TFE3 protein only when phosphorylated at Ser321.

    Species Reactivity:

    Human, Mouse, Rat

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser321 of human TFE3 protein. Antibodies are purified by peptide affinity chromatography.

    Background

    Transcription factor E3 (TFE3) is a member of a family of basic helix-loop-helix leucine zipper transcription factors that includes MITF, TFEB, TFE3, and TFEC. Members of this family form heterodimers with each other, bind the same DNA sequences, and undergo the same types of post-translational modifications, including sumoylation (1). Research studies indicate that TFE3 and other family members play roles in development, organelle biogenesis, nutrient sensing, autophagy, and energy metabolism (2,3). Additional studies report that TFE3 controls the gate for pluripotent cells to exit the state of pluripotency prior to differentiation (4). Translocations involving the TFE3 gene region have been identified in a number of tumors, including sporadic renal cell tumors. Several specific translocations that result in kidney cancer and involve the TFE3 gene have been described and characterized in detail (5).

    Phosphorylation of TFE3 at Ser321 by mTOR leads to cytoplasmic retention analogous to Ser211 on TFEB (6). Dephosphorylation of TFEB and TFE3 by protein phosphatase 2A in response to oxidative stress leads to their nuclear translocation and activation (7).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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