Phospho-TSC2 Antibody Sampler Kit #8350

Protocols

• 3615: Western Blotting
• 3617: Western Blotting, Immunoprecipitation (Magnetic)
• 4308: Western Blotting, Immunoprecipitation (Magnetic), Immunofluorescence*, Flow
• 5584: Western Blotting, Immunoprecipitation (Magnetic)
• 7074: Western Blotting

The Phospho-TSC2 Antibody Sampler Kit provides an economical means to investigate protein folding within the cell. The kit contains enough primary and secondary antibodies to perform two western blot experiments per primary antibody.

Each antibody in the Phospho-TSC2 Antibody Sampler Kit recognizes only its specific target and does not cross-react with other family members unless otherwise indicated. Phospho-Tuberin/TSC2 (Ser1387) Antibody detects endogenous levels of tuberin protein only when phosphorylated at Ser1387. This antibody also cross-reacts with an unidentified 140 kDa protein.

Monoclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to carboxy-terminal residues of human tuberin protein or a synthetic phosphopeptide corresponding to residues surrounding Thr1462 of human tuberin protein. Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Ser939 or corresponding to residues surrounding Ser1387 of human tuberin protein. Polyclonal antibodies are purified by protein A and peptide affinity chromatography.

Tuberin is a product of the TSC2 tumor suppressor gene and an important regulator of cell proliferation and tumor development (1). Mutations in either TSC2 or the related TSC1 (hamartin) gene cause tuberous sclerosis complex (TSC), an autosomal dominant disorder characterized by development of multiple, widespread non-malignant tumors (2). Tuberin is directly phosphorylated at Thr1462 by Akt/PKB (3). Phosphorylation at Thr1462 and Tyr1571 regulates tuberin-hamartin complexes and tuberin activity (3-5). In addition, tuberin inhibits the mammalian target of rapamycin (mTOR), which promotes inhibition of p70 S6 kinase, activation of eukaryotic initiation factor 4E binding protein 1 (4E-BP1, an inhibitor of translation initiation), and eventual inhibition of translation (3,6,7).

1. Soucek, T. et al. (1998) Proc Natl Acad Sci U S A 95, 15653-8.
2. Sparagana, S.P. and Roach, E.S. (2000) Curr Opin Neurol 13, 115-9.
3. Manning, B.D. et al. (2002) Mol Cell 10, 151-62.
4. Aicher, L.D. et al. (2001) J Biol Chem 276, 21017-21.
5. Dan, H.C. et al. (2002) J Biol Chem 277, 35364-70.
6. Goncharova, E.A. et al. (2002) J Biol Chem 277, 30958-67.
7. Inoki, K. et al. (2002) Nat Cell Biol 4, 648-57.

Data sheet (88KB) MSDS PDF