Render Target: STATIC
Render Timestamp:
3/25/2025, 6:42:16 AM EDT
3/25/2025, 10:42:16 AM UTC
Commit: 8d93f7ebe45006d66c127727d817fc3f57c4fe9a
XML generation date: 2025-03-07 13:09:03.659
Product last modified at: 2025-01-01T09:04:43.964Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

Phospho-Tuberin/TSC2 (Tyr1571) Antibody #3614

Filter:
  • WB

    Supporting Data

    REACTIVITY M Mk
    SENSITIVITY Endogenous
    MW (kDa) 200
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • M-Mouse 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Phospho-Tuberin/TSC2 (Tyr1571) Antibody detects endogenous levels of tuberin only when phosphorylated at tyrosine 1571. This antibody does not detect tuberin phosphorylated at other sites.

    Species Reactivity:

    Mouse, Monkey

    The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.

    Species predicted to react based on 100% sequence homology:

    Human

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr1571 of human tuberin. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    Tuberin is a product of the TSC2 tumor suppressor gene and an important regulator of cell proliferation and tumor development (1). Mutations in either TSC2 or the related TSC1 (hamartin) gene cause tuberous sclerosis complex (TSC), an autosomal dominant disorder characterized by development of multiple, widespread non-malignant tumors (2). Tuberin is directly phosphorylated at Thr1462 by Akt/PKB (3). Phosphorylation at Thr1462 and Tyr1571 regulates tuberin-hamartin complexes and tuberin activity (3-5). In addition, tuberin inhibits the mammalian target of rapamycin (mTOR), which promotes inhibition of p70 S6 kinase, activation of eukaryotic initiation factor 4E binding protein 1 (4E-BP1, an inhibitor of translation initiation), and eventual inhibition of translation (3,6,7).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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